Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55780
Borycki AG, et al., Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development. 1999 Sep;126(18):4053-63
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1342692
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1M,N:
embryonic day 10.5
(more  )
Specimen
1M,N:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
1M
,
1N
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Expression is throughout the dermomyotome in the interlimb somites.
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Specimen
1O,P:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
1P
,
1O
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In mutant embryos, expression in the interlimb somites is restricted to the ventro-lateral domain of the somite.
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Specimen
2E:
embryonic day 10.5
(more )
Note: Transverse section at the interlimb level.
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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Transverse section at the interlimb level.
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Specimen
2F:
embryonic day 10.5; Shhtm1Chg/ Shhtm1Chg
(more )
Note: Transverse section at the interlimb level.
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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Transverse section at the interlimb level.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
2F
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Expression is restricted to the ventro-lateral domain.
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| TS17: trunk somite |
Present |
Regionally restricted |
2F
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Expression is restricted to the ventro-lateral domain.
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Specimen
2K:
embryonic day 10.5
(more )
Note: Transverse section at the level of the tail. This section shows a newly formed somite.
Specimen
2K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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Transverse section at the level of the tail. This section shows a newly formed somite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail somite |
Present |
Regionally restricted |
2K
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Expression is in the epaxial and hypaxial domain.
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Specimen
2L:
embryonic day 10.5; Shhtm1Chg/ Shhtm1Chg
(more )
Note: Transverse section at the level of the tail. This section shows a newly formed somite.
Specimen
2L:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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Transverse section at the level of the tail. This section shows a newly formed somite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail somite |
Present |
Uniform |
2L
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In mutants, the expression expands into the entire somite.
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Analysis Tools