Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56564
De Arcangelis A, et al., Synergistic activities of alpha3 and alpha6 integrins are required during apical ectodermal ridge formation and organogenesis in the mouse. Development. 1999 Sep;126(17):3957-68
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1342445
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Gene symbol:
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Lama5
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Gene name:
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laminin, alpha 5
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Antibody:
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4G6
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
5d:
embryonic day 10.5
(more  )
Specimen
5d:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
5d
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Expression is in the basal lamina underlying ridge and non-ridge ectoderm.
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Specimen
5h:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
Itga6tm1Egl/Itga6tm1Egl
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Weak |
Regionally restricted |
5h
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In mutants, the signal is less intense than in wild-type and it is irregular.
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb ectoderm |
Present |
Regionally restricted |
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Expression is in the basal lamina underlying ridge and non-ridge ectoderm.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
Itga6tm1Egl/Itga6tm1Egl
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb ectoderm |
Weak |
Regionally restricted |
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In mutants, the signal is less intense than in wild-type and it is irregular.
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Analysis Tools
