Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:54746
Matise MP, et al., Ventral midline cells are required for the local control of commissural axon guidance in the mouse spinal cord. Development. 1999 Aug;126(16):3649-59
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:1340853
|
Gene symbol:
|
Cntn2
|
Gene name:
|
contactin 2
|
|
Results
|
Specimen
2.C:
embryonic day 11.0
(more )
Specimen
2.C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
2C
|
Expression is detected on commissural axons.
|
Specimen
2.F:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gli2tm1Alj/Gli2tm1Alj
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5.C:
embryonic day 11.0
(more )
Specimen
5.C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
5C
|
Expression is seen on commissural axons projecting ventrally towards the floor plate, but decreases as axons cross the midline in the ventral commissure; no expression is seen on longitudinal axons in the ventral funiculus.
|
Specimen
5.D:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gli2tm1Alj/Gli2tm1Alj
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
5D
|
As in wild-type, expression is seen on commissural axons projecting ventrally towards the midline. In contrast to wild-type, expression was also seen on the (rare) longitudinally directed commissural axon.
|
Specimen
5.G:
embryonic day 11.0
(more )
Specimen
5.G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
5G
|
Expression is seen on commissural axons projecting ventrally towards the floor plate, but decreases as axons cross the midline in the ventral commissure; no expression is seen on longitudinal axons in the ventral funiculus.
|
Specimen
5.H:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gli2tm1Alj/Gli2tm1Alj
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
5H
|
As in wild-type, expression is seen on commissural axons projecting ventrally towards the midline. In contrast to wild-type, expression was also seen on the (rare) longitudinally directed commissural axon.
|
Specimen
5.I:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gli2tm1Alj/Gli2tm1Alj
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: future spinal cord |
Present |
Regionally restricted |
5I
|
As in wild-type, expression is seen on commissural axons projecting ventrally towards the midline. In contrast to wild-type, expression was also seen on the (rare) longitudinally directed commissural axon.
|
Specimen
not shown:
embryonic day 10.0-11.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0-11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
|
Expression was detected on dorsal commissural cells in the spinal cord and hindbrain.
|
TS17: hindbrain |
Present |
Regionally restricted |
|
Expression was detected on dorsal commissural cells in the spinal cord and hindbrain.
|
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gli2tm1Alj/Gli2tm1Alj
|
Age:
|
embryonic day 10.0-11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
|
Expression was detected on dorsal commissural cells in the spinal cord and hindbrain. Expression pattern was similar to that in the wild-type.
|
TS17: hindbrain |
Present |
Regionally restricted |
|
Expression was detected on dorsal commissural cells in the spinal cord and hindbrain. Expression pattern was similar to that in the wild-type.
|
|