Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50462
Dassule HR, et al., Analysis of epithelial-mesenchymal interactions in the initial morphogenesis of the mammalian tooth. Dev Biol. 1998 Oct 15;202(2):215-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340493
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Gene symbol:
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Wnt10b
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Gene name:
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wingless-type MMTV integration site family, member 10B
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Results
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Specimen
1B:
embryonic day 11.0
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: 1st branchial arch mandibular component |
Weak |
Regionally restricted |
1B
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Wnt10b is expressed at the mesial tip of the jaw in a short, thin strip of epithelial cells that extends distally. In all cases, expression was symmetrical on either side of the median sulcus.
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| TS18: tongue |
Absent |
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1B
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Specimen
1H:
embryonic day 11.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: mandible primordium |
Strong |
Regionally restricted |
1H
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The midline expression extends further distally. Expression is upregulated relative to E11.0. Highest levels of expression are mesially and distally with an apparent downregulation in between.
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| TS19: tongue |
Absent |
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1H
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Specimen
1N:
embryonic day 12.0
(more )
Specimen
1N:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mandible primordium |
Present |
Regionally restricted |
1N
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Expression is completely downregulated in diastema. Expression occurs at midline in incisor domains and further distally in molar primordia. Double in situs of Wnt10a/Shh and Wnt10b/Shh confirm expression in either the same or intermixed cell populations.
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| TS20: tongue |
Absent |
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1N
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Specimen
1T:
embryonic day 12.0
(more )
Note: Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
1T:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mandible primordium |
Strong |
Regionally restricted |
1T
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Wnt10b is expressed at the tip of the tooth epithelium.
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Specimen
2C:
embryonic day 13.5
(more )
Note: Bud stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
2C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Bud stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower jaw molar epithelium |
Strong |
Regionally restricted |
2C
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Wnt10b is strongly expressed at the tip of the invaginating epithelium. The distribution of Wnt10b appears to be asymmetric, although this might be an artifact of the plane of section.
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Specimen
2D:
embryonic day 14.5
(more )
Note: Cap stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
2D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Cap stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
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