Antibody:	anti-CRABP I
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The fixation is 4% paraformaldehyde/4% TCA/ 10% isopropyl alcohol. There are two Crabp1 antibodies. Either antibody may have been used in this assay. See MGI:4460229 for details about the second antibody.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	2D	Expression is in streams of neural crest that is migrating in rhombomeres 4 and 6, populating the branchial arches and heart.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	2E	Expression is in a stripe in the future rhombencephalon when the neural folds are still open.

Structure	Level	Pattern	Image	Note
TS15: future midbrain roof plate	Present		4A
TS15: rhombomere 1	Absent		4A
TS15: rhombomere 3	Absent		4A
TS15: rhombomere 2	Weak		4A
TS15: rhombomere 4	Strong		4A
TS15: rhombomere 6	Strong		4A
TS15: 1st branchial arch	Present	Regionally restricted	4A
TS15: 2nd branchial arch	Present	Regionally restricted	4A
TS15: heart	Present	Regionally restricted	4A
TS15: migrating neural crest	Present	Regionally restricted	4A	Expression is also in neural crest cells migrating in three streams. The rostral stream from rhombomere 2 to branchial arch 1. The middle stream from rhombomere 4 to branchial arch 2. The caudal stream from rhombomere 6 to branchial arch 3 and the heart.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component mesenchyme derived from neural crest	Weak		4E
TS16: 1st branchial arch maxillary component mesenchyme derived from neural crest	Weak		4E
TS16: 2nd branchial arch mesenchyme derived from neural crest	Strong		4E