Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339659
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Gene symbol:
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Fgf4
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Gene name:
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fibroblast growth factor 4
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Probe:
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Fgf4 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3J:
embryonic day 11.5; En1tm2Alj/ En1tm2Alj
(more  )
Note: Limb-bud stage (stage 5/6).
Specimen
3J:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: apical ectodermal ridge |
Present |
Regionally restricted |
3J
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Expression is in the dorsal rim and a small region of the ventral rim of the bifurcated apical ectodermal ridge.
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Analysis Tools
