Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1339658
|
|
Gene symbol:
|
Jag1
|
|
Gene name:
|
jagged 1
|
|
Probe:
|
Jag1 cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
3G:
embryonic day 11.5
(more  )
Note: Limb-bud stage (stage 5/6).
Specimen
3G:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Limb-bud stage (stage 5/6).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb mesenchyme |
Present |
Regionally restricted |
3G
|
Expression is in the progress zone underlying the apical ectodermal ridge.
|
Specimen
3H:
embryonic day 11.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 5/6).
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
En1tm2Alj/En1tm2Alj
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Limb-bud stage (stage 5/6).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb mesenchyme |
Present |
Regionally restricted |
3H
|
In mutants, the region of expression is expanded to include cells underlying the ectopic apical ectodermal ridge.
|
|
Analysis Tools