Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339653
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Gene symbol:
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Fgf4
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Gene name:
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fibroblast growth factor 4
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Probe:
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Fgf4 probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date (E10.5-11.5).
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Results
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Specimen
2K:
embryonic day 10.5; En1tm1Alj/En1 +
(more  )
Note: Limb-bud stage (stage 3/4). There are discrepancies between the figure and the figure legend as to the genotype of the embryo.
Specimen
2K:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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En1tm1Alj/En1+
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4). There are discrepancies between the figure and the figure legend as to the genotype of the embryo.
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Specimen
2L:
embryonic day 10.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 3/4).
Specimen
2L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4).
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Analysis Tools