Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339620
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Gene symbol:
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Dlx2
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Gene name:
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distal-less homeobox 2
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Probe:
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Dlx2 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Some assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date.
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Results
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Specimen
1E:
embryonic day 9.5
(more  )
Note: Limb-bud stage (stage 1).
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1E
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Expression is in the distal half of the ventral limb bud.
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Specimen
2C:
embryonic day 9.5-10.5
(more )
Note: Limb-bud stage (stage 1/2).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5-10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1/2).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: limb ectoderm |
Present |
Regionally restricted |
2C
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Restricted proximoventral expression.
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Specimen
2D:
embryonic day 9.5-10.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 1/2).
Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 9.5-10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1/2).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: limb ectoderm |
Present |
Regionally restricted |
2D
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In mutants, the expression domain is broader than in wild-type limbs, a marked expansion of the ventroproximal expression domain.
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Specimen
2G:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3/4).
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4).
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Specimen
3A:
embryonic day 11.5
(more )
Note: Limb-bud stage (stage 5/6).
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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Specimen
3B:
embryonic day 11.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 5/6).
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: apical ectodermal ridge |
Present |
Regionally restricted |
3B
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Broader expression in mutants. There are two distinct rims of stained ectoderm separated by a less intense stained region of epithelium. Expression is wider anteriorly than posteriorly.
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Specimen
Not shown:
embryonic day 9.0
(more )
Note: Limb-bud stage (stage 0.25-0.5).
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 0.25-0.5).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud ectoderm |
Present |
Regionally restricted |
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Expression is throughout the ventral ectoderm.
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Specimen
Not shown:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3).
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3).
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Specimen
Not shown:
embryonic day 10.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 3/4).
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4).
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Analysis Tools