Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339617
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En1 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Some assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date.
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Note: Limb-bud stage (stage 1).
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1A
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Expression is in a restricted proximoventral position.
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Specimen
1B:
embryonic day 9.5
(more )
Note: Limb-bud stage (stage 1).
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1B
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Expression is in a restricted proximoventral position.
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Specimen
1C:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3).
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 3).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: apical ectodermal ridge |
Present |
Regionally restricted |
1C
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Expression extends over the entire ventral limb including the ventral apical ectodermal ridge.
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| TS17: limb ectoderm |
Present |
Regionally restricted |
1C
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Expression extends over the entire ventral limb including the ventral apical ectodermal ridge.
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Analysis Tools