Probe:	Kit probe3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		6A
TS17: 2nd branchial arch	Present		6A
TS17: future spinal cord	Present		6A
TS17: limb	Present		6A
TS17: tail paraxial mesenchyme	Present		6A
TS17: trunk paraxial mesenchyme	Present		6A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Strong		6B	Expression levels in this mutant are higher than in wild-type embryos.
TS17: 2nd branchial arch	Strong		6B	Expression levels in this mutant are higher than in wild-type embryos.
TS17: limb	Strong		6B	Expression levels in this mutant are higher than in wild-type embryos.
TS17: tail paraxial mesenchyme	Strong		6B	Expression levels in this mutant are higher than in wild-type embryos.
TS17: trunk paraxial mesenchyme	Strong		6B	Expression levels in this mutant are higher than in wild-type embryos.
TS17: forelimb bud apical ectodermal ridge	Present		6B	This represents ectopic expression in this mutant.
TS17: hindlimb bud apical ectodermal ridge	Present		6B	This represents ectopic expression in this mutant.
TS17: trunk mesenchyme	Present	Regionally restricted	6B	This represents ectopic expression in this mutant. Expression is in the newly formed mesenchymal dermis is at the mid-trunk level.
TS17: future spinal cord	Present	Regionally restricted	6B	In this mutant, expression is enhanced in the dorsal aspect of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Absent		6D +/+
TS19: neural tube	Present		6D +/+

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Strong		6D Ph/+	The expression levels are abberant in these mutant embryos.
TS19: limb	Strong		6D Ph/+	The expression levels are abberant in these mutant embryos.
TS19: neural tube	Strong		6D Ph/+	The expression levels are abberant in these mutant embryos.
TS19: outer ear	Strong		6D Ph/+	The expression levels are abberant in these mutant embryos.
TS19: footplate apical ectodermal ridge	Present		6D Ph/+	This mutant shows ectopic expression in the AER.
TS19: handplate apical ectodermal ridge	Present		6D Ph/+	This mutant shows ectopic expression in the AER.

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Strong		6D Ph/Ph	The expression levels are abberant in these mutant embryos.
TS19: limb	Strong		6D Ph/Ph	The expression levels are abberant in these mutant embryos.
TS19: neural tube	Strong		6D Ph/Ph	The expression levels are abberant in these mutant embryos.
TS19: outer ear	Strong		6D Ph/Ph	The expression levels are abberant in these mutant embryos.
TS19: footplate apical ectodermal ridge	Strong		6D Ph/Ph	This mutant shows ectopic expression in the AER.
TS19: handplate apical ectodermal ridge	Strong		6D Ph/Ph	This mutant shows ectopic expression in the AER.

Structure	Level	Pattern	Image	Note
TS21: limb	Absent		7_+/+	Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: outer ear	Absent		7_+/+	Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: skin	Absent		7_+/+	Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: spinal cord	Absent		7_+/+	Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.

Structure	Level	Pattern	Image	Note
TS21: limb	Present	Regionally restricted	7_Ph/+	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: outer ear	Present	Regionally restricted	7_Ph/+	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: skin	Present	Regionally restricted	7_Ph/+	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: spinal cord	Present	Regionally restricted	7_Ph/+	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.

Structure	Level	Pattern	Image	Note
TS21: limb	Present	Regionally restricted	7_Ph/Ph	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: outer ear	Present	Regionally restricted	7_Ph/Ph	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: skin	Present	Regionally restricted	7_Ph/Ph	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS21: spinal cord	Present	Regionally restricted	7_Ph/Ph	In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.

Structure	Level	Pattern	Image	Note
TS20: limb	Absent			Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS20: skin	Absent			Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS20: spinal cord	Absent			Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS20: tail mesenchyme derived from neural crest	Absent			Expression is absent in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted		In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS20: skin	Present	Regionally restricted		In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS20: spinal cord	Present	Regionally restricted		In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.
TS20: tail mesenchyme derived from neural crest	Present	Regionally restricted		In mutant embryos ectopic expression is seen in the mesenchyme of the ear pinna, limb buds, dermal mesenchyme and neural tube.