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Gene Expression Data
Assay Details
Assay
Reference: J:34184 Yang XM, et al., Expression of the met receptor tyrosine kinase in muscle progenitor cells in somites and limbs is absent in Splotch mice. Development. 1996 Jul;122(7):2163-71
Assay type: RNA in situ
MGI Accession ID: MGI:1338933
Gene symbol: Met
Gene name: met proto-oncogene
Probe: pmet SC1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: For the exact breeding scheme of mutant mice, see the original reference.
Results
Specimen not shown: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo Absent

Specimen not shown: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: embryo Absent

Specimen 1A: embryonic day 9.5 (more )
Note: 30 somites.
Structure Level Pattern Image Note
TS15: trunk somite Present Regionally restricted 1A Expression is in the ventral portion of the somites at the level of the forelimb buds and in the dorsal tip of a few rostral somites.
TS15: tail Present 1A

Specimen 1B: embryonic day 9.75 (more )
Note: 36 somites.
Structure Level Pattern Image Note
TS15: trunk somite Present Regionally restricted 1B There is no expression in the ventral tip at the level of the forelimb. Expression is in the ventral region of thoracic, hindlimb, and tail somites. There is expression in the dorsal tip of the somites.
TS15: forelimb bud Present Regionally restricted 1B

Specimen 1C: embryonic day 10.0 (more )
Note: 45 somites.
Structure Level Pattern Image Note
TS16: trunk somite Present Regionally restricted 1C There is no expression in the ventral tips at the level of the hindlimb.
TS16: hindlimb bud Present 1C

Specimen 1E: embryonic day 10.0 (more )
Note: 45 somites. This is a close-up of the embryo in 1C.
Structure Level Pattern Image Note
TS16: tail somite Present Regionally restricted 1E Expression is in the dorsal and ventral tips of the somites.
TS16: trunk somite Present Regionally restricted 1E Expression is in the dorsal and ventral tips of the somites.
TS16: hindlimb bud Present 1E

Specimen 1I: embryonic day 10.0 (more )
Note: 45 somites.
Structure Level Pattern Image Note
TS16: forelimb bud mesenchyme Present 1I

Specimen 1J: embryonic day 11.0 (more )
Note: 53 somites.
Structure Level Pattern Image Note
TS18: forelimb bud Present Regionally restricted 1J Expression is on the ventral side.

Specimen 1K: embryonic day 11.5 (more )
Note: 58 somites.
Structure Level Pattern Image Note
TS19: handplate Present Regionally restricted 1K Expression is on the dorsal side in the digit-forming area.

Specimen 1L: embryonic day 11.5 (more )
Note: 58 somites.
Structure Level Pattern Image Note
TS19: handplate Present Regionally restricted 1L Expression is on the ventral side in the digit-forming area.

Specimen 2C: embryonic day 9.75 (more )
Note: 36 somites. The section is from the level of the forelimb.
Structure Level Pattern Image Note
TS15: trunk dermomyotome Present Regionally restricted 2C Expression is in the ventrolateral tip.

Specimen 2D: embryonic day 9.75 (more )
Note: 36 somites. The section is from the level of the forelimb.
Structure Level Pattern Image Note
TS15: trunk mesenchyme Present Regionally restricted 2D Expressing cells localized to the lateral tip of the dermomyotome appeared to detach from the somite domain and accumulate in clusters at the body-limb junction, and then further invade the limb.

Specimen 2E: embryonic day 10.0 (more )
Note: 40 somites. The section is from the level of the forelimb.
Structure Level Pattern Image Note
TS16: forelimb bud Present 2E Expressing cells localized to the lateral tip of the dermomyotome appeared to detach from the somite domain and accumulate in clusters at the body-limb junction, and then further invade the limb.
TS16: trunk dermomyotome Present 2E Expression is in the dorsal-medial tip.

Specimen 3B: embryonic day 10.5 (more )
Note: 46-50 somites.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3B Expression is in a subapical domain on the dorsal and ventral limb side.

Specimen 4A: embryonic day 11.0 (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present 4A
TS18: hindlimb bud mesenchyme Present 4A
TS18: trunk somite Present Regionally restricted 4A Expression is in the ventral tip of thoracic and caudal somites and in the dorsal tip of nearly all somites.

Specimen 4B: embryonic day 11.0 (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present 4B
TS18: hindlimb bud mesenchyme Present 4B
TS18: trunk somite Present Regionally restricted 4B Expression is in the ventral tip of thoracic and caudal somites.

Specimen 4C: embryonic day 11.0 (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present 4C
TS18: hindlimb bud mesenchyme Present 4C

Specimen 4D: embryonic day 11.0; Pax3Sp-2H/Pax3+ (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present 4D Expression in this mutant is the same as in wild-type embryos.
TS18: hindlimb bud mesenchyme Present 4D Expression in this mutant is the same as in wild-type embryos.
TS18: trunk somite Present Regionally restricted 4D Expression in this mutant is the same as in wild-type embryos.

Specimen 4E: embryonic day 11.0; Pax3Sp-2H/Pax3+ (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present 4E Expression in this mutant is the same as in wild-type embryos.
TS18: hindlimb bud mesenchyme Present 4E Expression in this mutant is the same as in wild-type embryos.
TS18: trunk somite Present Regionally restricted 4E Expression in this mutant is the same as in wild-type embryos.

Specimen 4F: embryonic day 11.0; Pax3Sp-2H/Pax3+ (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present 4F Expression in this mutant is the same as in wild-type embryos.
TS18: hindlimb bud mesenchyme Present 4F Expression in this mutant is the same as in wild-type embryos.
TS18: trunk somite Present Regionally restricted 4F Expression in this mutant is the same as in wild-type embryos.

Specimen 4G: embryonic day 11.0; Pax3Sp-2H/Pax3Sp-2H (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Absent 4G
TS18: hindlimb bud mesenchyme Absent 4G
TS18: trunk somite Trace Regionally restricted 4G In these mutants, expression is missing in the ventral tip of thoracic somites and is severely decreased in the dorsal tip.

Specimen 4H: embryonic day 11.5; Pax3Sp-2H/Pax3Sp-2H (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS20: forelimb Absent 4H
TS20: somite Trace Regionally restricted 4H In these mutants, expression is missing in the ventral tip of thoracic somites and is severely decreased in the dorsal tip.

Specimen 4I: embryonic day 11.5; Pax3Sp-2H/Pax3Sp-2H (more )
Note: 48 somites.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Absent 4I


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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory