Antibody:	anti-Cx43
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS7: epiblast	Weak	Spotted	1A	Expression is primarily in the primitive endoderm.
TS7: extraembryonic visceral endoderm	Weak	Spotted	1A	Expression is primarily in the primitive endoderm.
TS28: decidua	Strong	Spotted	1A

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present	Spotted	1B
TS10: embryo mesoderm	Present	Spotted	1B
TS10: extraembryonic endoderm	Present	Spotted	1B
TS10: embryo ectoderm	Strong	Spotted	1B	Expression is most prevalent in the ectoderm.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Uniform	2A	Labeled gap junctions appear to be homogeneously distributed within the ectoderm.
TS12: foregut diverticulum	Present	Spotted	2A
TS12: heart	Present	Spotted	2A
TS12: head mesenchyme	Present	Regionally restricted	2A	The anterior mesoderm is negative with the exception of the heart primordium.
TS12: trunk mesenchyme	Present	Regionally restricted	2A	The anterior mesoderm is negative with the exception of the heart primordium.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Uniform	2B	Labeled gap junctions appear to be homogeneously distributed within the ectoderm.
TS12: head somite	Present	Spotted	2B	Staining was in the posterior regions and the somites.
TS12: trunk mesenchyme	Present	Spotted	2B	Staining was in the posterior regions and the somites.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present	Spotted	3A
TS16: telencephalon	Present	Regionally restricted	3A	Expression is in a circumscribed region.

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis infundibulum	Strong	Spotted	3B
TS20: remnant of Rathke's pouch	Absent		3B

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Strong	Regionally restricted	3C	Expression is in the future epiphysis (pineal gland).
TS21: embryo mesenchyme	Strong	Regionally restricted	3C	Expression is in the mesenchyme surrounding the diencephalon.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Strong	Spotted	4A	Expression in lens placode is stronger than in the surrounding surface ectoderm.
TS16: optic cup	Present	Spotted	4A
TS16: eye	Present	Regionally restricted	4A	Expression is in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Strong	Spotted	4B
TS21: retina	Present	Regionally restricted	4B	Expression is in the sensory layer in the region that will become the iris.
TS21: lens anterior epithelium	Present		4D
TS21: lens fiber	Present		4D

Structure	Level	Pattern	Image	Note
TS20: cornea epithelium	Present	Spotted	4C
TS20: lens vesicle anterior epithelium	Present	Spotted	4C
TS20: perioptic mesenchyme	Present	Spotted	4C
TS20: lens vesicle	Present	Regionally restricted	4C	Expression is between lens fibers.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Spotted	5
TS19: limb ectoderm	Weak	Spotted	5
TS19: limb mesenchyme	Absent		5

Structure	Level	Pattern	Image	Note
TS16: primitive ventricle	Trace	Spotted	6A

Structure	Level	Pattern	Image	Note
TS20: heart ventricle	Present	Regionally restricted	6B	Expression is along the trabeculae that extend inward from the ventricular wall.