T RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:45340 Friedrich GA, et al., The secretory protein Sec8 is required for paraxial mesoderm formation in the mouse. Dev Biol. 1997 Dec 15;192(2):364-74
Assay type:	RNA in situ
MGI Accession ID:	MGI:1338188
Gene symbol:	T
Gene name:	brachyury, T-box transcription factor T

Probe:	brachyury probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5B +/+ ©

Specimen 5B +/+: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Uniform	5B +/+

5B -/- ©

Specimen 5B -/-: embryonic day 7.5; Exoc4^{Gt(SABetageo4)Sor}/Exoc4^{Gt(SABetageo4)Sor} (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	5B -/-	In mutant embryos expression is confined to the posterior-most regions. This is similar to 6.5dpc wild-type embryos.
TS11: primitive streak	Present	Regionally restricted	5B -/-	In mutant embryos expression is confined to the posterior-most regions. This is similar to 6.5dpc wild-type embryos.

5C +/+ ©

Specimen 5C +/+: embryonic day 8.25 (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Uniform	5C +/+	Expression is in the node and the notochord.

5C -/- ©

Specimen 5C -/-: embryonic day 8.25; Exoc4^{Gt(SABetageo4)Sor}/Exoc4^{Gt(SABetageo4)Sor} (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Uniform	5C -/-	Expression in this mutant is the same as in wild-type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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