Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29276
Acampora D, et al., Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development. 1995 Oct;121(10):3279-90
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1337962
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA7
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4f,i:
embryonic day 9.5; Otx2tm1Pas/ Otx2tm1Pas
(more  )
Note: Transverse section showing the strongest phenotype.
Specimen
4f,i:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm1Pas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Transverse section showing the strongest phenotype.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm1Pas
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Absent |
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In wild-type embryos, expression is seen in the midline mesoderm of the head process.
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Analysis Tools
