Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:29276
Acampora D, et al., Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development. 1995 Oct;121(10):3279-90
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1337948
|
|
Gene symbol:
|
Hoxb1
|
|
Gene name:
|
homeobox B1
|
|
Probe:
|
Hoxb1 cDNA4
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
4c,d:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Otx2tm1Pas/Otx2tm1Pas
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 5 |
Present |
|
4c/d
|
The distance between the rostral end of the embryo and the anterior border of expression is strongly reduced relative to wild-type mice.
|
Specimen
4g,h:
embryonic day 9.5; Otx2tm1Pas/ Otx2tm1Pas
(more  )
Note: Sagittal section of an embryo with a severe phenotype.
Specimen
4g,h:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Otx2tm1Pas/Otx2tm1Pas
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Sagittal section of an embryo with a severe phenotype.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
4g/h
|
Two broad domains of expression flank the segmented mesoderm, reminiscent of expression in wild-type embryos at 8.0dpc.
|
Specimen
6i,j:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * DBA/2
|
|
Mutant Allele(s):
|
Otx2tm1Pas/Otx2tm1Pas
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Sagittal section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Strong |
Regionally restricted |
6i/j
|
Expression is in the posteriorly forming mesoderm.
|
|
Analysis Tools
