Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30825
Sporle R, et al., Severe defects in the formation of epaxial musculature in open brain (opb) mutant mouse embryos. Development. 1996 Jan;122(1):79-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336664
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1E:
embryonic day 10.5
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
Regionally restricted |
1E
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Expression is weak in the dorsal and ventral dermomyotome and strong in the elongating somitic bud.
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| TS17: somite |
Present |
Regionally restricted |
1E
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Expression is weak in the dorsal and ventral dermomyotome and strong in the elongating somitic bud.
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Specimen
1F:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
Regionally restricted |
1F
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Expression is drastically reduced in the dorsal demomyotome. Expression in the ventral dermomyotome and somitic bud is normal.
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| TS17: somite |
Present |
Regionally restricted |
1F
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Expression is drastically reduced in the dorsal demomyotome. Expression in the ventral dermomyotome and somitic bud is normal.
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
5B
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Expression occurred in an extremely narrow band in mutants compared to wild type.
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| TS15: somite |
Present |
Regionally restricted |
5B
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Expression in early somites did not extend as far dorsally as in the wild type and was absent at the anterior and posterior margins.
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Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
5D
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Expression occurred in an extremely narrow band compared to wild type, and there were gaps in this band.
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Analysis Tools