Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54643
Storm EE, et al., GDF5 coordinates bone and joint formation during digit development. Dev Biol. 1999 May 1;209(1):11-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336330
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Gene symbol:
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Gdf5
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Gene name:
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growth differentiation factor 5
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Probe:
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Gdf5 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4C:
embryonic day 12.5
(more  )
Specimen
4C:
(close  )
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Genetic Background:
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BALB/cJ
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4C
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Expression is in the digital ray in a domain of approximately 30-35 cell diameters distal to the developing metatarsal.
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Specimen
4G:
embryonic day 13.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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BALB/cJ
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4G
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Expression is in a domain of approximately 15-20 cell diameters in the region of the presumptive metatarsophalangeal joint.
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Specimen
4K:
(close )
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Genetic Background:
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involves: BALB/cJ
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Mutant Allele(s):
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Gdf5bp-3J/Gdf5bp-3J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4K
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Expression is in a broad region distal to the metatarsal. This pattern is similar to that seen in 12.5dpc wild-type limbs.
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Specimen
4N:
embryonic day 14.5
(more )
Specimen
4N:
(close )
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Genetic Background:
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BALB/cJ
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4N
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Expression is in the metatarsophalangeal joint and in the 1st and 2nd interphalangeal joints.
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Specimen
4R:
(close )
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Genetic Background:
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involves: BALB/cJ
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Mutant Allele(s):
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Gdf5bp-3J/Gdf5bp-3J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4R
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Expression is expanded to a region that encompases the region of the proximal and medial phalanges in a pattern that is complememtary to Col2a1.
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