Gene Expression Data
Assay Details
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Assay
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Reference:
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J:23774
Chen WH, et al., Genesis and prevention of spinal neural tube defects in the curly tail mutant mouse: involvement of retinoic acid and its nuclear receptors RAR-beta and RAR-gamma. Development. 1995 Mar;121(3):681-91
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1335180
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Gene symbol:
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Rarb
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Gene name:
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retinoic acid receptor, beta
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Probe:
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mRAR-beta
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1A RAR[B] ct/ct:
embryonic day 10.0; Grhl3ct/ Grhl3ct
(more  )
Note: This embryo has 27 somites. This is a category 1/2 mutant.
Specimen
1A RAR[B] ct/ct:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 27 somites. This is a category 1/2 mutant.
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Specimen
1C RAR[B]:
embryonic day 10.0
(more )
Note: This embryo has 27 somites.
Specimen
1C RAR[B]:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 27 somites.
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Specimen
2B:
embryonic day 10.5; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 29 somites. This is a category 1/2 mutant. The level of this section is shown in figure 1A.
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 29 somites. This is a category 1/2 mutant. The level of this section is shown in figure 1A.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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2B
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There is no expression in the neuropore.
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Specimen
2C:
embryonic day 10.5; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 29 somites. This is a category 1/2 mutant. The level of this section is shown in figure 1A.
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 29 somites. This is a category 1/2 mutant. The level of this section is shown in figure 1A.
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Specimen
2D:
embryonic day 10.5; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 29 somites. This is a category 1/2 mutant. The level of this section is shown in figure 1A.
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 29 somites. This is a category 1/2 mutant. The level of this section is shown in figure 1A.
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Specimen
2E:
embryonic day 10.5
(more )
Note: This embryo has 28 somites. The level of this section is shown in figure 1A.
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 28 somites. The level of this section is shown in figure 1A.
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Specimen
2G:
embryonic day 10.5; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 28 somites. This is a category 3/4 mutant. The level of this section is shown in 2F.
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 28 somites. This is a category 3/4 mutant. The level of this section is shown in 2F.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindgut |
Weak |
Regionally restricted |
2G
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Expression in the hindgut endoderm is much lower than in wild-type embryos.
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| TS17: tail nervous system |
Weak |
Regionally restricted |
2G
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Neuroepithelial expression is lower than in wild-type embryos.
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Specimen
2H:
embryonic day 10.5; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 28 somites. This is a category 3/4 mutant. The level of this section is shown in 2F.
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 28 somites. This is a category 3/4 mutant. The level of this section is shown in 2F.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindgut |
Weak |
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2H
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Expression is much less than in a wild-type embryo.
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| TS17: tail nervous system |
Weak |
Regionally restricted |
2H
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Neuroepithelial expression is lower than in the wild-type embryo.
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Specimen
5B:
embryonic day 10.0
(more )
Note: This embryo has 28 somites. The level of this section is shown in 5A.
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 28 somites. The level of this section is shown in 5A.
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Specimen
5C:
embryonic day 10.0; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 31 somites. The level of this section is shown in Fig. 5A. This embryo is a vehicle-treated control.
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 31 somites. The level of this section is shown in Fig. 5A. This embryo is a vehicle-treated control.
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Specimen
5E:
embryonic day 10.0; Grhl3ct/ Grhl3ct
(more )
Note: This embryo has 31 somites. The level of this section is shown in Fig. 5A. This embryo is a vehicle-treated control.
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo has 31 somites. The level of this section is shown in Fig. 5A. This embryo is a vehicle-treated control.
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