Gene Expression Data
Assay Details
Assay
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Reference:
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J:23137
Kwee L, et al., Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development. 1995 Feb;121(2):489-503
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1334373
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Gene symbol:
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Itga4
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Gene name:
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integrin alpha 4
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Antibody:
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9C10
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2E:
embryonic day 8.5
(more )
Specimen
2E:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS13: extraembryonic component |
Present |
Regionally restricted |
2E
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Expression is in the chorionic epithelium juxtaposed to the Vcam1-expressing regions of the allantois. Authors also note expression in the yolk sac, but it is not visible in the figure.
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TS13: heart |
Absent |
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2E
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Specimen
3C:
embryonic day 8.75
(more )
Specimen
3C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3F:
embryonic day 11.5
(more )
Specimen
3F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: visceral pericardium |
Present |
Regionally restricted |
3F
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Expression is in the cells encasing the myocardium adjacent to the layer that shows high expression of Vcam1.
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TS19: endocardial cushion |
Present |
Regionally restricted |
3F
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Expression is in mesenchymal cells.
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Specimen
4D:
(close )
Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Vcam1tm1Roml/Vcam1tm1Roml
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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