Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43311
Peichel CL, et al., The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning. Development. 1997 Sep;124(18):3481-92
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333590
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Gene symbol:
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Hoxd13
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Gene name:
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homeobox D13
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Probe:
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probe B
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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See the Materials and Methods for details of the possible strain backgrounds.
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Results
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Specimen
2A +/+ upper:
embryonic day 11.0
(more  )
Specimen
2A +/+ upper:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: limb |
Present |
Regionally restricted |
2A +/+ upper
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Expression is restricted to the distal autopod (digits) of the limb.
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Specimen
2A +/+ lower:
embryonic day 11.0
(more )
Specimen
2A +/+ lower:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
2A +/+ lower
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Expression is restricted to the distal autopod (digits) of the limb.
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Specimen
2A Ul/+ upper:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
2A Ul/+ upper:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/Lnpk+
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: limb |
Present |
Regionally restricted |
2A Ul/+ upper
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In embryos carrying the Ul allele, ectopic expression is seen in the zeugopod and in the autopod (digits) there is reduced expression.
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Specimen
2A Ul/+ lower:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
2A Ul/+ lower:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/Lnpk+
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
2A Ul/+ lower
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In embryos carrying the Ul allele, ectopic expression is seen in the zeugopod and in the autopod (digits) there is reduced expression.
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Specimen
2A Ul/Ul upper:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
2A Ul/Ul upper:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/LnpkUl
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: limb |
Present |
Regionally restricted |
2A Ul/Ul upper
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In embryos carrying the Ul allele, ectopic expression is seen in the zeugopod and in the autopod (digits) there is reduced expression.
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Specimen
2A Ul/UL lower:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
2A Ul/UL lower:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/LnpkUl
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
2A Ul/Ul lower
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In embryos carrying the Ul allele, ectopic expression is seen in the zeugopod and in the autopod (digits) there is reduced expression.
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Specimen
5C +/+:
embryonic day 11.0
(more )
Specimen
5C +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C Ul/+:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
5C Ul/+:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/Lnpk+
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C Ul/Ul:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
5C Ul/Ul:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/LnpkUl
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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