Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52751
Tuttle R, et al., Defects in thalamocortical axon pathfinding correlate with altered cell domains in Mash-1-deficient mice. Development. 1999 May;126(9):1903-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333517
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Gene symbol:
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Nkx2-1
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Gene name:
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NK2 homeobox 1
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Probe:
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Nkx-2.1 probe-4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6.A:
embryonic day 13.5
(more  )
Specimen
6.A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6.B:
embryonic day 13.5
(more )
Specimen
6.B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: medial ganglionic eminence |
Present |
Regionally restricted |
6B
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Expression is seen in the mantle zone, particularly in the globus pallidus; central region has most, if not all, expressing cells and surrounding area has scattered positive cells.
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Specimen
6.C:
embryonic day 13.5
(more )
Specimen
6.C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: medial ganglionic eminence |
Present |
Regionally restricted |
6C
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Expression is seen in the mantle zone, particularly in the globus pallidus; central region has most, if not all, expressing cells and surrounding area has scattered positive cells.
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Specimen
6.E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
6E
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Size of expressing area is much reduced as compared to wild-type.
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Specimen
6.F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
6F
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Size of expressing area is much reduced as compared to wild-type.
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Specimen
6.G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
6G
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Size of expressing area is much reduced as compared to wild-type.
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Specimen
7.A:
embryonic day 13.5
(more )
Specimen
7.A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7.C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
7C
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There is no expression detectable at the diencephalic-telencephalic border where thalamocortical axons normally enter the ventral telencephalon; expression is present elsewhere, though.
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Specimen
7.E:
embryonic day 13.5
(more )
Specimen
7.E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools