Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30824
Grass S, et al., Alterations in somite patterning of Myf-5-deficient mice: a possible role for FGF-4 and FGF-6. Development. 1996 Jan;122(1):141-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333255
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Results
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Specimen
3A:
embryonic day 9.5
(more  )
Note: 24 somites.
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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24 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head somite |
Strong |
Regionally restricted |
3A
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Both wild-type and mutant embryos expressed high levels of Pax-1 mRNA in somites at the forelimb bud and at the interlimb level.
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| TS13: trunk somite |
Strong |
Regionally restricted |
3A
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Both wild-type and mutant embryos expressed high levels of Pax-1 mRNA in somites at the forelimb bud and at the interlimb level.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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24 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Strong |
Regionally restricted |
3B
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Both wild-type and mutant embryos expressed high levels of Pax-1 mRNA in somites at the forelimb bud and at the interlimb level.
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Specimen
3C:
embryonic day 9.5
(more )
Note: 24 somites. transverse section
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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24 somites. transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Regionally restricted |
3C
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Expression is present in sclerotomal cells.
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Specimen
3E:
embryonic day 9.5
(more )
Note: 24 somites. frontal section
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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24 somites. frontal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Regionally restricted |
3E
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Expression is present in sclerotomal cells.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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24 somites. transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Regionally restricted |
3D
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Expression in the mutant is as is wild type; it is present in sclerotomal cells.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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24 somites. frontal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Regionally restricted |
3F
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Expression in the mutant is as is wild type; it is present in sclerotomal cells.
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Specimen
not shown wt:
embryonic day 10.5
(more )
Note: 32 somites.
Specimen
not shown wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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32 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
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Pax-1 is uniformly expressed throughout the sclerotome.
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Specimen
not shown mutant:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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32 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
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Pax-1 is uniformly expressed throughout the sclerotome. A moderate extension of the region of expression towards the dermomyotome was seen in mutants.
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