Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:42444
Blackburn MR, et al., Genetically engineered mice demonstrate that adenosine deaminase is essential for early postimplantation development. Development. 1997 Aug;124(16):3089-97
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:1333006
|
|
Gene symbol:
|
Ada
|
|
Gene name:
|
adenosine deaminase
|
|
Antibody:
|
anti ADA
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
Fixation for this assay was 70% ethanol, 10% formalin, and 5% acetic acid.
|
|
|
Results
|
Specimen
1A embryo:
embryonic day 7.5
(more )
Specimen
1A embryo:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
1A mother:
embryonic day 7.5
(more )
Specimen
1A mother:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Strong |
|
1A
|
Expression was present in the secondary deciduum.
|
Specimen
2A embryo:
embryonic day 9.5
(more )
Note: This embryo may be either wild type or heterozygous for the Adatm1Mw allele.
Specimen
2A embryo:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
This embryo may be either wild type or heterozygous for the Adatm1Mw allele.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: extraembryonic component |
Strong |
Regionally restricted |
2A
|
Intense staining is seen in the giant trophoblast cells, the syncytiotrophoblasts of the developing labyrinthine zone and the spongiotrophoblasts of the junctional zone.
|
Specimen
2A mother:
embryonic day 9.5; Adatm1Mw/Ada +
(more )
Specimen
2A mother:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6 * FVB/N
|
|
Mutant Allele(s):
|
Adatm1Mw/Ada+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Strong |
|
2A
|
Expression was present in the secondary deciduum.
|
Specimen
2B embryo:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6 * FVB/N
|
|
Mutant Allele(s):
|
Adatm1Mw/Adatm1Mw
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
2B mother:
embryonic day 9.5; Adatm1Mw/Ada +
(more )
Specimen
2B mother:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6 * FVB/N
|
|
Mutant Allele(s):
|
Adatm1Mw/Ada+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Strong |
|
2B
|
Expression was present in the secondary deciduum.
|
|