Ptafr RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:53301 Stojanov T, et al., Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. Biol Reprod. 1999 Mar;60(3):674-82
Assay type:	RT-PCR
MGI Accession ID:	MGI:1332843
Gene symbol:	Ptafr
Gene name:	platelet-activating factor receptor

Probe:	Ptafr-pC, Ptafr-pD
Visualized with:	Ethidium bromide
Assay notes:	This assay was a nested PCR where MGI:1332800 was used as the internal primer pair.

Results

Image: 4_PAF-receptor

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	235 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
M	Control
Liv	P adult	TS28: liver	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
UE	P adult (a)	TS28: secondary oocyte	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Female
1e	E0.5 (b)	TS1: 1-cell stage conceptus	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
1L	E0.5 (c)	TS1: 1-cell stage conceptus	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
2c	E1.0 (d)	TS2: 2-cell stage conceptus	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
4c	E2.0 (e)	TS3: 4-cell stage conceptus	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
8c	E2.5 (f)	TS3: 8-cell stage conceptus	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
bl	E3.5 (g)	TS4: blastocyst-stage conceptus	Present	Not Specified; total RNA	Swiss	Tyr^c/Tyr^c	Not Specified
Lane 10	Control

Notes:
(a) 17 hours post-hCG.
(b) 21 hours post-hCG.
(c) 27 hours post-hCG.
(d) 42 hours post-hCG.
(e) early 4-cell stage. 52 hours post-hCG.
(f) 8-cell stage. 66 hours post-hCG.
(g) blastocyst. 90 hours post-hCG.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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