Gene Expression Data
Assay Details
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Assay
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Reference:
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J:40390
Sasaki H, et al., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development. 1997 Apr;124(7):1313-22
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1332605
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli cDNA5
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
4C:
embryonic day 8.5
(more  )
Note: 9-11 somites.
Specimen
4C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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9-11 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future spinal cord neural plate |
Strong |
Regionally restricted |
4C
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Gli transcripts were abundant in the ventral region of the neural tube/plate and gradually lost dorsally. In the posterior part, its expression overlaps with HNF-3b.
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| TS13: neural tube |
Strong |
Regionally restricted |
4C
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Gli transcripts were abundant in the ventral region of the neural tube/plate and gradually lost dorsally. In the posterior part, its expression overlaps with HNF-3b.
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| TS13: neural tube floor plate |
Absent |
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4C
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In the anterior part, Gli expression is excluded from the floor plate.
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Specimen
4H:
embryonic day 8.5
(more )
Note: 9-11 somites.
Specimen
4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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9-11 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future spinal cord neural plate |
Strong |
Regionally restricted |
4H
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Gli transcripts were abundant in the ventral region of the neural tube/plate and gradually lost dorsally. In the posterior part, its expression overlaps with HNF-3b.
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| TS13: neural tube |
Strong |
Regionally restricted |
4H
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Gli transcripts were abundant in the ventral region of the neural tube/plate and gradually lost dorsally. In the posterior part, its expression overlaps with HNF-3b.
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Specimen
not shown:
embryonic day 8.5
(more )
Note: 9-11 somites.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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9-11 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Strong |
Regionally restricted |
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The ventral boundary of Gli expression apparently coincides with the Shh boundary.
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| TS13: future prosencephalon |
Strong |
Regionally restricted |
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The ventral boundary of Gli expression apparently coincides with the Shh boundary.
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Specimen
4M:
embryonic day 9.5
(more )
Specimen
4M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Graded |
4M
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Gli transcripts were abundant in the ventral region of the neural tube/plate and gradually lost dorsally.
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| TS15: tail neural plate |
Present |
Graded |
4M
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Gli transcripts were abundant in the ventral region of the neural tube/plate and gradually lost dorsally.
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