Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49658
Thomas T, et al., A signaling cascade involving endothelin-1, dHAND and msx1 regulates development of neural-crest-derived branchial arch mesenchyme. Development. 1998 Aug;125(16):3005-14
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331698
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Gene symbol:
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Hand1
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Gene name:
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heart and neural crest derivatives expressed 1
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Probe:
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eHAND probe2
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 9.0
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1B:
embryonic day 10.0
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: 1st branchial arch |
Present |
Regionally restricted |
1B
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Expression was limited to the distal portion of the arches, with dHAND expression being slightly broader than eHAND.
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| TS16: 2nd branchial arch |
Present |
Regionally restricted |
1B
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Expression was limited to the distal portion of the arches, with dHAND expression being slightly broader than eHAND.
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Specimen
not shown:
embryonic day 10.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: 1st branchial arch |
Present |
Regionally restricted |
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There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
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| TS16: 1st branchial arch mesenchyme derived from neural crest |
Present |
Regionally restricted |
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There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
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| TS16: 2nd branchial arch |
Present |
Regionally restricted |
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There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
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| TS16: 2nd branchial arch mesenchyme derived from neural crest |
Present |
Regionally restricted |
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There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
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| TS16: 1st branchial arch ectoderm |
Absent |
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| TS16: 2nd branchial arch ectoderm |
Absent |
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| TS16: migrating neural crest |
Absent |
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Specimen
2D:
embryonic day 10.0
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Edn1tm1Utj/Edn1tm1Utj
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: primitive ventricle |
Present |
Regionally restricted |
2H
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eHAND expression in the left ventricle was unchanged.
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| TS16: 1st branchial arch |
Weak |
Regionally restricted |
2H
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eHAND expression in the first and second branchial arches was much lower and restricted to a small region in Edn1-null embryos in comparison to wild type.
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| TS16: 2nd branchial arch |
Weak |
Regionally restricted |
2H
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eHAND expression in the first and second branchial arches was much lower and restricted to a small region in Edn1-null embryos in comparison to wild type.
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