Gene Expression Data
Assay Details
Assay
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Reference:
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J:47
Niswander L, et al., Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse. Development. 1992 Mar;114(3):755-68
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331198
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Gene symbol:
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Fgf3
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Gene name:
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fibroblast growth factor 3
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Probe:
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c.28
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Probe preparation:
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labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2F:
embryonic day 6.25
(more )
Note: Early postimplantation embryo;shortly before the beginning of gastrulation; prior to primitive streak formation.
Specimen
2F:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 6.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early postimplantation embryo;shortly before the beginning of gastrulation; prior to primitive streak formation.
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Specimen
not shown:
embryonic day 6.5-6.75
(more )
Note: Early streak stage.
Specimen
not shown:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 6.5-6.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early streak stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo mesoderm |
Present |
|
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Transcripts are detected in mesoderm that has migrated into the extraemryonic region, as well as in embryonic mesoderm.
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Specimen
3F:
embryonic day 7.25
(more )
Note: Mid-streak stage. Transverse section.
Specimen
3F:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Mid-streak stage. Transverse section.
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Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo mesoderm |
Present |
Graded |
3F
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Transcripts continue to be expressed in cells exiting the streak; however the level of expression decreases in cells near the leading edge of each mesodermal wing.
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Specimen
3G/H:
embryonic day 6.75
(more )
Note: Early streak stage. Transverse section.
Specimen
3G/H:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early streak stage. Transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo mesoderm |
Present |
Uniform |
3G/H
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RNA is detected in embryonic mesoderm as it emerges along the length of the streak and spreads laterally around the epiblast.
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Specimen
3I/J:
embryonic day 7.5
(more )
Note: Late-streak stage. Transverse section.
Specimen
3I/J:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 7.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Late-streak stage. Transverse section.
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Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo mesoderm |
Present |
Regionally restricted |
3I/J
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Expression is detected in the embryo proper only in mesoderm that extends out from the primitive streak 1/2-3/4 of the distance around the epiblast, and is not detected in the anterolateral mesoderm.
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TS10: extraembryonic mesoderm |
Present |
Regionally restricted |
3I/J
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Expression remains detectable in mesoderm around the entire circumference of the exocoelomic cavity.
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