Probe:	Sox9 cDNA3
Probe preparation:	Antisense labelled with radioactivity RNA

Structure	Level	Pattern	Image	Note
TS20: middle ear mesenchyme	Present	Regionally restricted	1E	Expressed in the condensation surrounding the stapedial artery.

Structure	Level	Pattern	Image	Note
TS20: middle ear mesenchyme	Present	Regionally restricted	1H	Expressed in the condensation surrounding the stapedial artery.

Structure	Level	Pattern	Image	Note
TS21: stapes pre-cartilage condensation	Present		1K
TS21: otic capsule	Present		1K	Expressed in the otic capsule, but not in the oval window.

Structure	Level	Pattern	Image	Note
TS20: temporal bone petrous part	Present	Regionally restricted	1N	Expressed in the developing styloid process.

Structure	Level	Pattern	Image	Note
TS19: 2nd branchial arch	Present	Regionally restricted	3D , 3B/C	Expression is restricted to a central area in the branchial arch, and in the dorsal part shows a perforated appearance in the stapedial condensation, which lies in close proximity to the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: 2nd branchial arch	Present	Regionally restricted	3F/G , 3H	The altered expression was particularly evident in the dorsal part, where it was no longer close to the otic vesicle but more ventrally located, adjacent to the first pharyngeal pouch.