Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49817
Acampora D, et al., Visceral endoderm-restricted translation of otx1 mediates recovery of otx2 requirements for specification of anterior neural plate and normal gastrulation. Development. 1998 Dec;125(24):5091-104
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1321305
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Gene symbol:
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Lhx1
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Gene name:
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LIM homeobox protein 1
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Probe:
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Lim1 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
4J:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm1Pas
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early streak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Regionally restricted |
4J
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The expression was abnormally localized at the distal region of the embryo.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm1Pas
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Late streak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Absent |
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5H
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Lim1 transcripts were not detected in the axial mesoderm.
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| TS10: primitive streak |
Present |
Regionally restricted |
5H
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Lim1 was expressed posteriorly in mesoderm cells along the abnormal primitive streak.
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Analysis Tools
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