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Gene Expression Data
Assay Details
Assay
Reference: J:49252 Hardcastle Z, et al., The Shh signalling pathway in tooth development: defects in Gli2 and Gli3 mutants. Development. 1998 Aug;125(15):2803-11
Assay type: RNA in situ
MGI Accession ID: MGI:1320900
Gene symbol: Ptch1
Gene name: patched 1
Probe: Ptc cDNA3
Probe preparation: Antisense labelled with S35 RNA
Results
Specimen 1C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: mandibular process Present Regionally restricted 1C Ptc in the molars was restricted to the mesenchyme underlying the tooth thickening. In the mandibular and maxillary incisors, the domain of Ptc appeared to have extended more laterally but was still localized in the odontogenic region of the mandible.
TS19: maxillary process Present Regionally restricted 1C Ptc in the molars was restricted to the mesenchyme underlying the tooth thickening. In the mandibular and maxillary incisors, the domain of Ptc appeared to have extended more laterally but was still localized in the odontogenic region of the mandible.

Specimen 1I: embryonic day 12.5 (more )
Note: Early tooth bud stage.
Structure Level Pattern Image Note
TS20: mandibular process Present Regionally restricted 1I Ptc was expressed uniformly in the odontogenic epithelium and mesenchyme of the incisors, whereas in the molars the medial pattern of expression was maintained.
TS20: maxillary process Present Regionally restricted 1I Ptc was expressed uniformly in the odontogenic epithelium and mesenchyme of the incisors, whereas in the molars the medial pattern of expression was maintained.

Specimen 1O: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: lower jaw molar Present Regionally restricted 1O In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
TS21: lower jaw molar epithelium Present Regionally restricted 1O In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
TS21: upper jaw molar Present Regionally restricted 1O In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
TS21: upper jaw molar epithelium Present Regionally restricted 1O In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
TS21: lower jaw tooth Strong Regionally restricted 1O In the dental follicle, Ptc was expressed more strongly on the lingual side of the tooth germs.
TS21: upper jaw tooth Strong Regionally restricted 1O In the dental follicle, Ptc was expressed more strongly on the lingual side of the tooth germs.
TS21: lower jaw incisor Strong Regionally restricted 1O In the incisors, Ptc was also expressed most strongly on the lingual side of the tooth germs.
TS21: upper jaw incisor Strong Regionally restricted 1O In the incisors, Ptc was also expressed most strongly on the lingual side of the tooth germs.

Specimen 1U: embryonic day 14.5 (more )
Note: Cap stage.
Structure Level Pattern Image Note
TS22: lower jaw incisor Present Regionally restricted 1U The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
TS22: lower jaw molar Present Regionally restricted 1U The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
TS22: upper jaw incisor Present Regionally restricted 1U The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
TS22: upper jaw molar Present Regionally restricted 1U The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
TS22: enamel organ Absent 1A' , 1U

Specimen 8A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: lower jaw molar Present 8A Ptc is normally expressed in molar tooth germs.
TS21: upper jaw molar Present 8A Ptc is normally expressed in molar tooth germs.

Specimen 8C: embryonic day 14.0; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS22: upper jaw molar epithelium Absent 8C Ptc was downregulated in the epithelium.
TS22: lower jaw molar Present 8C
TS22: upper jaw molar Present 8C

Specimen 8E: embryonic day 13.5; Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS21: lower jaw molar Strong 8E
TS21: upper jaw molar Strong 8E

Specimen 8G: embryonic day 13.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3+ (more )
Structure Level Pattern Image Note
TS21: lower jaw molar Present 8G
TS21: upper jaw molar Present 8G
TS21: lower jaw molar epithelium Moderate 8G Ptc expression was slightly weaker in the epithelial component.
TS21: upper jaw molar epithelium Moderate 8G Ptc expression was slightly weaker in the epithelial component.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory