Gene Expression Data
Assay Details
Assay
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Reference:
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J:49252
Hardcastle Z, et al., The Shh signalling pathway in tooth development: defects in Gli2 and Gli3 mutants. Development. 1998 Aug;125(15):2803-11
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1320900
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Probe:
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Ptc cDNA3
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS19: mandibular process |
Present |
Regionally restricted |
1C
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Ptc in the molars was restricted to the mesenchyme underlying the tooth thickening. In the mandibular and maxillary incisors, the domain of Ptc appeared to have extended more laterally but was still localized in the odontogenic region of the mandible.
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TS19: maxillary process |
Present |
Regionally restricted |
1C
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Ptc in the molars was restricted to the mesenchyme underlying the tooth thickening. In the mandibular and maxillary incisors, the domain of Ptc appeared to have extended more laterally but was still localized in the odontogenic region of the mandible.
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Specimen
1I:
embryonic day 12.5
(more )
Note: Early tooth bud stage.
Specimen
1I:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Early tooth bud stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: mandibular process |
Present |
Regionally restricted |
1I
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Ptc was expressed uniformly in the odontogenic epithelium and mesenchyme of the incisors, whereas in the molars the medial pattern of expression was maintained.
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TS20: maxillary process |
Present |
Regionally restricted |
1I
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Ptc was expressed uniformly in the odontogenic epithelium and mesenchyme of the incisors, whereas in the molars the medial pattern of expression was maintained.
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Specimen
1O:
embryonic day 13.5
(more )
Specimen
1O:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS21: lower jaw molar |
Present |
Regionally restricted |
1O
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In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
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TS21: lower jaw molar epithelium |
Present |
Regionally restricted |
1O
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In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
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TS21: upper jaw molar |
Present |
Regionally restricted |
1O
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In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
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TS21: upper jaw molar epithelium |
Present |
Regionally restricted |
1O
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In molars, Ptc was expressed in the dental papilla and also in regions of the dental epithelium.
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TS21: lower jaw tooth |
Strong |
Regionally restricted |
1O
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In the dental follicle, Ptc was expressed more strongly on the lingual side of the tooth germs.
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TS21: upper jaw tooth |
Strong |
Regionally restricted |
1O
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In the dental follicle, Ptc was expressed more strongly on the lingual side of the tooth germs.
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TS21: lower jaw incisor |
Strong |
Regionally restricted |
1O
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In the incisors, Ptc was also expressed most strongly on the lingual side of the tooth germs.
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TS21: upper jaw incisor |
Strong |
Regionally restricted |
1O
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In the incisors, Ptc was also expressed most strongly on the lingual side of the tooth germs.
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Specimen
1U:
embryonic day 14.5
(more )
Note: Cap stage.
Specimen
1U:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Cap stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: lower jaw incisor |
Present |
Regionally restricted |
1U
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The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
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TS22: lower jaw molar |
Present |
Regionally restricted |
1U
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The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
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TS22: upper jaw incisor |
Present |
Regionally restricted |
1U
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The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
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TS22: upper jaw molar |
Present |
Regionally restricted |
1U
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The pattern of expression of Ptc in the molars and incisors resembled that seen at E13.5.
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TS22: enamel organ |
Absent |
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1A'
,
1U
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Specimen
8A:
embryonic day 13.5
(more )
Specimen
8A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
8C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Specimen
8E:
(close )
Genetic Background:
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involves: C3H * CD-1
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
8G:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj Gli3Xt-J/Gli3+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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