Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49322
Zuniga A, et al., Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development. 1999 Jan;126(1):13-21
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1314736
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Gene symbol:
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Hoxb8
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Gene name:
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homeobox B8
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Probe:
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Hoxb-8 probe2
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Probe preparation:
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labelled with digoxigenin RNA
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Assay notes:
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All results were confirmed by also analyzing ld<In2>/Xt<H> intercrosses.
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Results
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Specimen
5a:
embryonic day 9.25
(more  )
Note: 23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
Specimen
5a:
(close  )
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Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
5a
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Expression is restricted to the posterior half of all limb buds. Expression in limb buds is shifted about 1.5 to 2 somites more anterior in comparison to the trunk.
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| TS15: trunk paraxial mesenchyme |
Present |
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5a
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Specimen
5b:
embryonic day 9.25; Fmn1ld-Is(17;In2)1Gso/ Fmn1ld-Is(17;In2)1Gso
(more )
Note: 23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
Specimen
5b:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Mutant Allele(s):
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Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
5b
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Expression is restricted to the posterior half of all limb buds. Expression in limb buds is shifted about 1.5 to 2 somites more anterior in comparison to the trunk.
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| TS15: trunk paraxial mesenchyme |
Present |
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5b
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Specimen
5c:
embryonic day 9.25; Gli3Xt-J/ Gli3Xt-J
(more )
Note: 23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
Specimen
5c:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
5c
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Expression is restricted to the posterior half of all limb buds. Expression in limb buds is shifted about 1.5 to 2 somites more anterior in comparison to the trunk.
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| TS15: trunk paraxial mesenchyme |
Present |
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5c
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Specimen
5d:
(close )
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Genetic Background:
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involves: C57BL/6 * SJL
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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23-25 somites. All embryos are age-matched, but the slight variations in limb-bud size are due to slight variations in outgrowth at this stage and not linked to the genotype.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
5d
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Expression is restricted to the posterior half of all limb buds. Expression in limb buds is shifted about 1.5 to 2 somites more anterior in comparison to the trunk.
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| TS15: trunk paraxial mesenchyme |
Present |
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5d
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Analysis Tools