Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:49322 Zuniga A, et al., Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development. 1999 Jan;126(1):13-21
Assay type:	RNA in situ
MGI Accession ID:	MGI:1314733
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh cDNA2
Probe preparation:	labelled with digoxigenin RNA
Assay notes:	All results were confirmed by also analyzing ld<ln2>/Xt<H> intercrosses.

Results

5e ©

Specimen 5e: embryonic day 10.25; Gli3^Xt-J/Gli3^Xt-J (more

)
Note: 32-34 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present	Regionally restricted	5e	At this developmental stage, expression in Xt/Xt mutants is indistinguishable from wild-type limb buds.

5f ©

Specimen 5f: embryonic day 10.25; Fmn1^{ld-Is(17;In2)1Gso}/Fmn1^{ld-Is(17;In2)1Gso} (more

)
Note: 32-34 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present	Regionally restricted	5f	Expression is activated at the posterior margin, but not upregulated.

5g ©

Specimen 5g: embryonic day 10.25; Gli3^Xt-J/Gli3^Xt-J, Fmn1^{ld-Is(17;In2)1Gso}/Fmn1^{ld-Is(17;In2)1Gso} (more

)
Note: 32-34 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Trace	Regionally restricted	5g	Expression was low and more posterior (g) or mispositioned to the limb-bud apex (h). Shh levels were much lower or not detectable in most other Xt;ld mutants and could not be reliably detected before E10.25.
TS16: forelimb bud mesenchyme	Trace	Regionally restricted	5g	Expression was never detected in the anterior half of the limb-bud mesenchyme.

5h ©

Specimen 5h: embryonic day 10.25; Gli3^Xt-J/Gli3^Xt-J, Fmn1^{ld-Is(17;In2)1Gso}/Fmn1^{ld-Is(17;In2)1Gso} (more

)
Note: 32-34 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Trace	Regionally restricted	5h	Expression was low and more posterior (g) or mispositioned to the limb-bud apex (h). Shh levels were much lower or not detectable in most other Xt;ld mutants and could not be reliably detected before E10.25
TS16: forelimb bud mesenchyme	Trace	Regionally restricted	5h	Expression was never detected in the anterior half of the limb-bud mesenchyme.

Specimen not shown: embryonic day 10.25 (more

)
Note: 32-34 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present	Regionally restricted

6a ©

Specimen 6a: embryonic day 11.0; Gli3^Xt-J/Gli3^Xt-J (more

)
Note: ~40 somites.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	6a	Expression is comparable to wild-type hindlimb buds at this developmental stage.

Specimen not shown: embryonic day 11.0; Gli3^Xt-J/Gli3^Xt-J (more

)
Note: ~40 somites.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Weak	Regionally restricted		Weak ectopic anterior expession is apparent around embryonic day 11 in forelimb buds.

Specimen not shown: embryonic day 11.0 (more

)
Note: ~40 somites.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted

6b ©

Specimen 6b: embryonic day 11.0; Fmn1^{ld-Is(17;In2)1Gso}/Fmn1^{ld-Is(17;In2)1Gso} (more

)
Note: ~40 somites.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Weak	Regionally restricted	6b	Expression remains proximal at low levels.

6c ©

Specimen 6c: embryonic day 11.0; Gli3^Xt-J/Gli3^Xt-J, Fmn1^{ld-Is(17;In2)1Gso}/Fmn1^{ld-Is(17;In2)1Gso} (more

)
Note: ~40 somites.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	6c	Xt;ld double homozygote with posterior expression.

6d ©

Specimen 6d: embryonic day 11.0; Gli3^Xt-J/Gli3^Xt-J, Fmn1^{ld-Is(17;In2)1Gso}/Fmn1^{ld-Is(17;In2)1Gso} (more

)
Note: ~40 somites.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	6d	Xt;ld double homozygote with apical expression.

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