Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49164
Ding Q, et al., Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice. Development. 1998 Jul;125(14):2533-43
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1278437
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Antibody:
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anti PAX6 (DSHB)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
6A:
embryonic day 10.5
(more  )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
6A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Embryo was fixed in PBS containing 3.7% formaldehyde.
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Specimen
6B:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Embryo was fixed in PBS containing 3.7% formaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
6B
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A large population of expressing cells could be found at the ventral midline in the rostral part of the hindbrain. In the caudal part of the hindbrain and the spinal cord, only a few Pax6-positive cells were located at the ventral midline.
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| TS17: neural tube |
Moderate |
Regionally restricted |
6B
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A slight reduction of the number of expressing cells was consistently observed in the dorsal part of the mutant neural tube.
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| TS17: tail neural tube |
Moderate |
Regionally restricted |
6B
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A slight reduction of the number of expressing cells was consistently observed in the dorsal part of the mutant neural tube.
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Specimen
not shown:
embryonic day 10.5
(more )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Embryo was fixed in PBS containing 3.7% formaldehyde.
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Specimen
not shown:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Embryo was fixed in PBS containing 3.7% formaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
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There was a ventral shift of the expression domain in both the mutant hindbrain and spinal cord.
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| TS17: tail future spinal cord |
Present |
Regionally restricted |
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There was a ventral shift of the expression domain in both the mutant hindbrain and spinal cord.
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Specimen
6C:
embryonic day 10.5
(more )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Embryo was fixed in PBS containing 3.7% formaldehyde.
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Specimen
6D:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
|
Embedding:
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Paraffin
|
|
Note:
|
Embryo was fixed in PBS containing 3.7% formaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
6D
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A large population of expressing cells could be found at the ventral midline in the rostral part of the hindbrain. In the caudal part of the hindbrain and the spinal cord, only a few Pax6-positive cells were located at the ventral midline.
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| TS17: neural tube |
Present |
Regionally restricted |
6D
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A slight reduction of the number of expressing cells was consistently observed in the dorsal part of the mutant neural tube.
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| TS17: tail neural tube |
Present |
Regionally restricted |
6D
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A slight reduction of the number of expressing cells was consistently observed in the dorsal part of the mutant neural tube.
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Specimen
6E:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: Embryo was fixed in PBS containing 3.7% formaldehyde.
Specimen
6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Embryo was fixed in PBS containing 3.7% formaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
6E
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A large population of expressing cells could be found at the ventral midline in the rostral part of the hindbrain. In the caudal part of the hindbrain and the spinal cord, only a few Pax6-positive cells were located at the ventral midline.
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Analysis Tools