Probe:	Bmp4 cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent
TS9: extraembryonic component	Absent
TS28: decidua	Present

Structure	Level	Pattern	Image	Note
TS11: allantois	Present
TS11: amnion	Present
TS28: decidua	Present

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present	Regionally restricted	1A/B	Expression is localized to the mesoderm and definitive endoderm in the primitive streak region at the very posterior of the embryo and in the ventral mesoderm caudal to the last somite.
TS13: amnion	Present		1A/B

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present	Regionally restricted	1C/D	Expression is localized to the mesoderm and definitive endoderm in the primitive streak region at the very posterior of the embryo and in the ventral mesoderm caudal to the last somite.
TS13: amnion	Present		1C/D

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present	Regionally restricted	1E/F	Expression is seen in the ventral and lateral mesoderm.
TS14: mesenchyme derived from somatopleure	Present	Regionally restricted	1E/F	Expression is seen in the ventral and lateral mesoderm.
TS14: mesenchyme derived from splanchnopleure	Present	Regionally restricted	1E/F	Expression is seen in the ventral and lateral mesoderm.

Structure	Level	Pattern	Image	Note
TS13: allantois	Strong			The authors state they observe high levels of hybridization in the allantois.
TS13: heart	Present			The authors state that transcripts are present in the myocardium of the early heart.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial pouch	Present	Regionally restricted	2A	Transcripts are localized to the dorsal ectoderm of the first branchial pouch.
TS14: inner ear	Present	Regionally restricted	2A	Transcripts are localized to the posterior half of the otic vesicles.
TS14: embryo	Present		2A	Transcripts are seen in the anterior half of the pocket between the frontonasal mass and the first branchial arch.
TS14: trunk mesenchyme	Present	Regionally restricted	2A	Hybridization is seen in the mesenchymal tissue of the flank in the vicinity of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Present	Regionally restricted	2B	Transcripts are localized to the neuroepithelium of the diencephalon that is destined to associate with Rathke's pouch and form the posterior pituitary gland.
TS14: embryo	Present	Regionally restricted	2B	Transcripts are seen in the anterior half of the pocket between the frontonasal mass and the first branchial arch.
TS14: trunk mesenchyme	Present	Regionally restricted	2B	Transcripts are seen in the mesodermal tissue surrounding the developing gut and early lung bud.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial pouch	Present	Regionally restricted	2C	Transcripts are localized to the dorsal ectoderm of the first branchial pouch.
TS14: embryo	Present	Regionally restricted	2C	Transcripts are seen in the anterior half of the pocket between the frontonasal mass and the first branchial arch.
TS14: trunk mesenchyme	Present	Regionally restricted	2C	Transcripts are seen in the mesodermal tissue surrounding the developing gut and early lung bud.

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Present	Regionally restricted	3A/B	Expression was detected in the ventral diencephalon.

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Present	Regionally restricted	3C/D	Transcripts are seen in the ventral diencephalon.
TS14: heart	Present	Regionally restricted	3C/D	Transcripts are seen in the truncus arteriosus.
TS14: trunk mesenchyme	Present	Regionally restricted	3C/D	Transcripts are seen in the mesoderm surrounding the gut.
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	3C/D	Transcripts are seen in the lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS17: infundibular recess of 3rd ventricle	Present		3E/F
TS17: diencephalon floor plate	Present	Regionally restricted	3E/F	Transcripts are localized in the floorplate of the diencephalon adjacent to Rathke's pouch.

Structure	Level	Pattern	Image	Note
TS14: atrioventricular canal	Strong	Regionally restricted	6A/B	High levels of expression are seen in the outer myocardial layer of the developing atrio-ventricular canal.

Structure	Level	Pattern	Image	Note
TS17: heart	Strong	Regionally restricted	6C/D	High levels of expression are limited to the myocardial layer of the truncus arteriosus. Expression does not extend into the conus arteriosus.
TS17: atrioventricular canal	Absent		6C/D

Structure	Level	Pattern	Image	Note
TS17: frontal process ectoderm	Present	Regionally restricted		Expression can be localized in the distal ectoderm of the facial processes.
TS17: gut mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		7A/B
TS17: limb mesenchyme	Present	Graded	7A/B	Expression is present in the mesenchyme of the limb buds in an anterior to posterior gradient and a distal to proximal gradient. The expression level is 10 fold higher in anterior sections compared to posterior sections.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		7C/D
TS17: limb mesenchyme	Present	Graded	7C/D	Expression is present in the mesenchyme of the limb buds in an anterior to posterior gradient and a distal to proximal gradient. The expression level is 10 fold higher in anterior sections compared to posterior sections.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		7E/F
TS17: limb mesenchyme	Present	Graded	7E/F	Expression is present in the mesenchyme of the limb buds in an anterior to posterior gradient and a distal to proximal gradient. The expression level is 10 fold higher in anterior sections compared to posterior sections.

Structure	Level	Pattern	Image	Note
TS17: nasal pit epithelium	Present		8A/B	Expression is in the epithelium/ectoderm within and around the developing nasal pit.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Absent
TS19: frontal process mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS21: vibrissa dermal component	Strong	Regionally restricted	8E/F	Expression is high in the mesenchymal condensations that will form part of the whisker follicles.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present		8G/H	Bpm4 expression is seen in the facial mesenchyme.
TS21: vibrissa dermal component	Strong	Regionally restricted	8G/H	Expression is high in the mesenchymal condensations that will form part of the whisker follicles.

Structure	Level	Pattern	Image	Note
TS25: embryo	Present	Regionally restricted		Expression is seen in the secretory/sensory epithelium of the inner ear.