About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:16971 Barnes JD, et al., Embryonic expression of Lim-1, the mouse homolog of Xenopus Xlim-1, suggests a role in lateral mesoderm differentiation and neurogenesis. Dev Biol. 1994 Jan;161(1):168-78
Assay type: RNA in situ
MGI Accession ID: MGI:1270220
Gene symbol: Lhx1
Gene name: LIM homeobox protein 1
Probe: Lhx1 cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5A: embryonic day 6.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS9: primitive streak Present Graded 5A Expression is highest an anterior limit of primitive straek.

Specimen 5B: embryonic day 7.0 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS10: node Present 5B
TS10: embryo mesoderm Weak 5B
TS10: primitive streak Weak 5B

Specimen 5C: embryonic day 7.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS11: embryo mesoderm Present 5C
TS11: node Present 5C
TS11: notochordal plate Present 5C
TS11: notochordal process Present 5C
TS11: primitive streak Present 5C

Specimen 5D: embryonic day 6.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS9: embryo mesoderm Present Regionally restricted 5D Expression is present in the mesoderm of the primitive streak but not in the endoderm.
TS9: primitive streak Present Regionally restricted 5D Expression is present in the mesoderm of the primitive streak but not in the endoderm.
TS9: embryo endoderm Absent 5D

Specimen 6A: embryonic day 7.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS11: embryo mesoderm Present Regionally restricted 6A Expression is limited to the very posterior of the node and the lateral/intermediate mesoderm.
TS11: node Present Regionally restricted 6A Expression is limited to the very posterior of the node and the lateral/intermediate mesoderm.

Specimen 6B: embryonic day 8.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS13: intermediate mesenchyme Present 6B Expression is limited to the intermediate mesoderm.
TS13: lateral plate mesoderm Present 6B Expression is limited to the intermediate mesoderm.
TS13: notochord Absent 6B Text states that no expression is seen in the node or notochord.

Specimen 6C: embryonic day 9.0 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS14: nephrogenic cord Present Regionally restricted 6C

Specimen 6D: embryonic day 9.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS15: mesonephros Present Regionally restricted 6D right Strongest expression posterior to somite 17.
TS15: nephric duct Present Regionally restricted 6D right Strongest expression posterior to somite 17.
TS15: diencephalon Weak 6D right

Specimen 6E: embryonic day 10.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS17: diencephalon Present 6E
TS17: future spinal cord Present 6E
TS17: hindbrain Present 6E
TS17: mesonephros Present 6E
TS17: nephric duct Present 6E

Specimen 6F: embryonic day 10.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6F Expression in spinal cord is limited to dorsal spinal cord.
TS17: future spinal cord Present Regionally restricted 6F Expression in spinal cord is limited to dorsal spinal cord.
TS17: hindbrain Present Regionally restricted 6F Expression in spinal cord is limited to dorsal spinal cord.

Specimen 6G: embryonic day 9.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS15: nephric duct Present 6G Expression is restricted to the mesonephric duct.

Specimen 6H: embryonic day 10.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6H Expression is limited to lateral diencephalon.

Specimen 6I: embryonic day 10.5 (more )
Note: Specimen was fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 6I Expression is limited to the commissural neurons.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/30/2025
MGI 6.24
The Jackson Laboratory