Analysis Tools
mortality/aging
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• mice exhibit a low survival rate past weaning
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• mice exhibit shorter life spans than control littermates
• however, a few mice can survive up to the age of 7 weeks when kept on a 30 degrees Celcius warm pad starting from weaning
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growth/size/body
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• mice have a smaller physique than control littermates
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behavior/neurological
reproductive system
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• at 24 hrs hCG injection (hCG24), no polyhedral luteal cells are seen in corpora lutea
• instead, multiple large Graafian follicles are present in the periphery of ovaries; some, but not all, contain an oocyte with a partial or complete cumulus layer
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• at 5-7 weeks of age, no corpora lutea are observed in the absence of gonadotropin stimulation
• at 24 hrs hCG injection (hCG24), no vascularized corpora lutea are present even in ovaries that are capable of releasing one or multiple oocytes
• however, follicular development is qualitatively normal in unstimulated post-pubertal females
• no excessive atresia is detected in ovaries from P25 superovulated females by Caspase-3 staining at 16 hrs after hCG injection (hCG16)
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• at 5-7 weeks of age, 3 of 5 ovaries exhibit a varying degree of vascular congestion
• however, ovaries from P25 superovulated females show normal endothelial cell (PECAM1, aka CD31) staining at hCG16
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small ovary
(
J:215159
)
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• ovaries collected from P23-P31 females at the time of euthanasia 24 hrs after superovulation are smaller than in heterozygote control siblings
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small uterus
(
J:215159
)
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• uteri collected from P23-P31 females at the time of euthanasia 24 hrs after superovulation are smaller than in heterozygote control siblings
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• at hCG24, only 2 of 6 females tested had 1 oocyte each in one of their oviducts versus an average of 20.5 +/- 1.7 oocytes per ovary in heterozygote controls
• at 16 hrs after hCG injection (hCG16), only 1 oocyte was retrieved from one of 2 pre-pubertal females tested versus an average of 18.0 +/- 4.8 oocytes per ovary released from heterozygote controls
• however, at hCG24, two outlying females ovulated a normal range of oocytes
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cardiovascular system
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• at 5-7 weeks of age, 3 of 5 ovaries exhibit a varying degree of vascular congestion
• however, ovaries from P25 superovulated females show normal endothelial cell (PECAM1, aka CD31) staining 16 hrs after hCG injection
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homeostasis/metabolism
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• at hCG24, serum progesterone concentrations are significantly lower than in heterozygote controls, even in females that ovulate a normal range of oocytes
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endocrine/exocrine glands
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• at 24 hrs hCG injection (hCG24), no polyhedral luteal cells are seen in corpora lutea
• instead, multiple large Graafian follicles are present in the periphery of ovaries; some, but not all, contain an oocyte with a partial or complete cumulus layer
|
|
|
• at 5-7 weeks of age, no corpora lutea are observed in the absence of gonadotropin stimulation
• at 24 hrs hCG injection (hCG24), no vascularized corpora lutea are present even in ovaries that are capable of releasing one or multiple oocytes
• however, follicular development is qualitatively normal in unstimulated post-pubertal females
• no excessive atresia is detected in ovaries from P25 superovulated females by Caspase-3 staining at 16 hrs after hCG injection (hCG16)
|
|
|
• at 5-7 weeks of age, 3 of 5 ovaries exhibit a varying degree of vascular congestion
• however, ovaries from P25 superovulated females show normal endothelial cell (PECAM1, aka CD31) staining at hCG16
|
small ovary
(
J:215159
)
|
|
• ovaries collected from P23-P31 females at the time of euthanasia 24 hrs after superovulation are smaller than in heterozygote control siblings
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cellular
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• at 24 hrs hCG injection (hCG24), no polyhedral luteal cells are seen in corpora lutea
• instead, multiple large Graafian follicles are present in the periphery of ovaries; some, but not all, contain an oocyte with a partial or complete cumulus layer
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