Phenotypes Associated with This Genotype

Genotype
MGI:6400427

• Allelic Composition: Dpp4^em1Rba/Dpp4⁺
• Genetic Background: C57BL/6J-Dpp4^em1Rba

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

lung inflammation ( J:279834 )

• inflammatory infiltrates are seen in the lungs of mice infected with MERS-15 intranasally at day 3 post infection

decreased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:279834 )

• while mice support infection and replication of MERS-15, a mouse adapted MERS-CoV (MERS-0) via serial passage for 15 rounds through the lungs of heterozygous Dpp4^em1Rba mice, heterozygotes show 100% survival with MERS-15 infection compared to homozygotes which show high mortality

increased susceptibility to Coronaviridae infection ( J:279834 )

• mice support efficient infection and replication of the human Middle East respiratory syndrome coronavirus (MERS-CoV) strain HCoV-EMC/2012, camel strain Dromedary/Al-Hasa-KFU-HKU13/2013, and recombinant virus derived from a molecular infectious clone (icMERS) in the lungs which is not seen in wild-type controls

• mice infected with MERS-15 intranasally, a mouse adapted MERS-CoV (MERS-0) via serial passage for 15 rounds through the lungs of heterozygous Dpp4^em1Rba mice, show higher levels of MERS-15 replication in the lungs at 3 and 6 days post infection, while MERS-0 is cleared from the lungs by 6 days post infection and no replication is seen in wild-type mice

mortality/aging

decreased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:279834 )

• while mice support infection and replication of MERS-15, a mouse adapted MERS-CoV (MERS-0) via serial passage for 15 rounds through the lungs of heterozygous Dpp4^em1Rba mice, heterozygotes show 100% survival with MERS-15 infection compared to homozygotes which show high mortality

respiratory system

lung hemorrhage ( J:279834 )

• severe hemorrhage in the lungs is seen in mice infected with MERS-15 intranasally at days 3 and 6 post infection

pulmonary alveolar edema ( J:279834 )

• mice infected with MERS-15 intranasally show intra-alveolar edema

lung inflammation ( J:279834 )

• inflammatory infiltrates are seen in the lungs of mice infected with MERS-15 intranasally at day 3 post infection

increased susceptibility to pulmonary hyaline membrane formation ( J:279834 )

• mice infected with MERS-15 intranasally show hyaline membrane formation at day 6 post infection

respiratory distress ( J:279834 )

• mice infected with MERS-15 intranasally show increased enhanced pause (Penh), a measure reflecting airway obstruction/restriction due to debris in the airway, and increased midtidal expiratory flow (EF50), representing the flow rate at which 50% of tidal volume is expelled in a single breath, up to day 6 post infection indicating severe respiratory distress

• mice infected with MERS-15 intranasally show pathology associated with severe acute respiratory distress, including hyaline membrane formation, intra-alveolar edema, perivascular cuffing and severe inflammation at day 6 post infection

cardiovascular system

lung hemorrhage ( J:279834 )

• severe hemorrhage in the lungs is seen in mice infected with MERS-15 intranasally at days 3 and 6 post infection

growth/size/body

increased susceptibility to weight loss ( J:279834 )

• mice infected with MERS-15 intranasally show 20-25% weight loss by days 6 post infection compared to mice infected with MERS-0 or wild-type mice injected with MERS-15

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:279834 )

N • blood glucose levels are normal

pulmonary alveolar edema ( J:279834 )

• mice infected with MERS-15 intranasally show intra-alveolar edema

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Middle East respiratory syndrome		DOID:0080642		J:279834