Phenotypes Associated with This Genotype

Genotype
MGI:5904844

• Allelic Composition: Mcoln2^{tm1e.1(KOMP)Wtsi}/Mcoln2^{tm1e.1(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6J * C57BL/6N
• Cell Lines: EPD0300_5_B06

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal chemokine level ( J:239875 )

• following LPS treatment (1 ug/ml) for 24 h, bone marrow-derived macrophages (BMDMs) show increased accumulation of CCL2 in the Golgi relative to LPS-treated wild-type cells, indicating altered trafficking of specific chemokines

abnormal chemokine secretion ( J:239875 )

• following LPS treatment for 24 h, production of several chemokines (CCL3, CCL5, and CXCL2) and of ICAM1 (CD54) is reduced in BMDM lysates relative to that in LPS-treated wild-type controls

• after LPS treatment, the amount of secreted CCL2 is significantly decreased in cell culture supernatants at all time points, as measured by ELISA

abnormal innate immunity ( J:239875 )

• mice exhibit impaired recruitment of peripheral macrophages in response to i.p. injections of LPS or live bacteria, suggesting an altered innate immune response

impaired macrophage chemotaxis ( J:239875 )

• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b⁺F4/80^dim peritoneal macrophages is significantly reduced relative to that in wild-type controls

• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 10⁷ CFU), the % of recruited CD11b⁺F4/80^dim macrophages is significantly reduced relative to that in wild-type controls

impaired neutrophil chemotaxis ( J:239875 )

• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G⁺CD16⁺ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls

• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 10⁷ CFU), migration of Ly6G⁺CD16⁺ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls

hematopoietic system

impaired macrophage chemotaxis ( J:239875 )

• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b⁺F4/80^dim peritoneal macrophages is significantly reduced relative to that in wild-type controls

• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 10⁷ CFU), the % of recruited CD11b⁺F4/80^dim macrophages is significantly reduced relative to that in wild-type controls

impaired neutrophil chemotaxis ( J:239875 )

• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G⁺CD16⁺ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls

• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 10⁷ CFU), migration of Ly6G⁺CD16⁺ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls

cellular

impaired macrophage chemotaxis ( J:239875 )

• at 8 h after i.p. injection of LPS (0.5 ug/g), the % of recruited CD11b⁺F4/80^dim peritoneal macrophages is significantly reduced relative to that in wild-type controls

• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 10⁷ CFU), the % of recruited CD11b⁺F4/80^dim macrophages is significantly reduced relative to that in wild-type controls

impaired neutrophil chemotaxis ( J:239875 )

• at 8 h after i.p. injection of LPS (0.5 ug/g), migration of Ly6G⁺CD16⁺ neutrophils into the intraperitoneal space is significantly decreased relative to that in wild-type controls

• at 8 h after i.p. injection of live enterotoxigenic E. coli (strain H10407) (5 x 10⁷ CFU), migration of Ly6G⁺CD16⁺ neutrophils into the intraperitoneal space is significantly delayed relative to that in wild-type controls

homeostasis/metabolism

abnormal chemokine level ( J:239875 )

• following LPS treatment (1 ug/ml) for 24 h, bone marrow-derived macrophages (BMDMs) show increased accumulation of CCL2 in the Golgi relative to LPS-treated wild-type cells, indicating altered trafficking of specific chemokines