Phenotypes Associated with This Genotype

Genotype
MGI:3833589

• Allelic Composition: Pold1^tm1Uchi/Pold1^tm1Uchi
• Genetic Background: C57BL/6-Pold1^tm1Uchi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Impaired proliferation of Pold1^tm1Uchi/Pold1^tm1Uchi blastocysts

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:144992 )

• no homozygotes detected among live births

• no homozygotes found at E7.5 or beyond

• E3.5 and E4.5 homozygotes indistinguishable from controls

embryo

absent inner cell mass proliferation ( J:144992 )

• inner cell mass from E3.5 embryos in culture fails to proliferate

inner cell mass degeneration ( J:144992 )

• inner cell mass from E3.5 embryos in culture begins to degenerate soon after appearing

• fragmented micronuclei present suggestive of apoptosis

• effects very noticeable after 3 days in culture but not after 1 day

abnormal trophoblast layer morphology ( J:144992 )

• trophoblast cells from E3.5 embryos in culture spread more slowly than those from controls

cellular

absent inner cell mass proliferation ( J:144992 )

• inner cell mass from E3.5 embryos in culture fails to proliferate