Phenotypes Associated with This Genotype

Genotype
MGI:3706513

• Allelic Composition: Tg(Ins2-Nos2)31Okam/0
• Genetic Background: involves: C57BL/6 * CD-1 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal pancreas morphology ( J:45520 )

abnormal pancreatic islet morphology ( J:45520 )

• transgenic islet cells show extensive DNA strand breaks compared to control islets

decreased pancreatic beta cell mass ( J:45520 )

• proportion of insulin-producing cell mass to total pancreatic cell mass is markedly reduced at 4 weeks of age

• however, no infiltration of macrophages or lymphocytes (insulitis) is observed

homeostasis/metabolism

hyperglycemia ( J:45520 )

• mice show hyperglycemia by one week of age with blood glucose above 400 mg/dl

decreased circulating insulin level ( J:45520 )

• serum insulin level (0.69 ng/dl) is decreased compared to controls (3.68 ng/dl)

abnormal nitric oxide homeostasis ( J:45520 )

• nitrite levels in medium, indicative of NO release, increase in a time-dependent manner, almost equivalent to the level in medium of Il-1beta stimulated control islets

• treatment with aminoguanidine (inhibitor of Nos2) prevents elevation of blood glucose levels and reduction in pancreatic insulin contents; reduction in insulin producing cell mass is reduced or improved with treatment

increased urine glucose level ( J:45520 )

• urine glucose levels are 200-500 mg/dl by 1 week of age

ketoaciduria ( J:45520 )

• urine in most mice tests positive for ketones

renal/urinary system

increased urine glucose level ( J:45520 )

• urine glucose levels are 200-500 mg/dl by 1 week of age

ketoaciduria ( J:45520 )

• urine in most mice tests positive for ketones

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:45520