Phenotypes Associated with This Genotype

Genotype
MGI:2175781

hm1

• Allelic Composition: Chek1^tm1Sje/Chek1^tm1Sje
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:62919 )

• die by E2.5-3.5

cellular

abnormal cell cycle checkpoint function ( J:62919 )

• initiation of mitosis occurred in mutant embryos despite exposure to DNA damage agents that normally arrest the cell cycle

increased embryonic tissue cell apoptosis ( J:62919 )

• massive DNA fragmentation during embryo degeneration

• TUNEL-positive cells are observed in mutant blastocysts, but not in control blastocysts

• ES cells made homozygous null appear apoptotic in culture

decreased cell proliferation ( J:62919 )

• mutant blastocysts stop proliferation and degenerate inside the zona pellucida

• failure of generation of homozygous ES cell colonies suggests that this gene product is required for cell proliferation

embryo

increased embryonic tissue cell apoptosis ( J:62919 )

• massive DNA fragmentation during embryo degeneration

• TUNEL-positive cells are observed in mutant blastocysts, but not in control blastocysts

• ES cells made homozygous null appear apoptotic in culture

failure of blastocyst to hatch from the zona pellucida ( J:62919 )

• a few cultured embryos do hatch, but do not contain an inner cell mass