Phenotypes associated with this allele

• Allele Symbol: Chodl^em1Feyu
• Allele Name: endonuclease-mediated mutation 1, Feng Yue
• Allele ID: MGI:8401759
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Chodl^em1Feyu/Chodl^em1Feyu Pax7^tm2.1(cre/ERT2)Fan/Pax7^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:8402601
cn2	Chodl^em1Feyu/Chodl^em1Feyu Myod1^tm2.1(icre)Glh/Myod1^+	involves: 129S6/SvEvTac * C57BL/6J	MGI:8402570

Genotype
MGI:8402601

cn1

• Allelic Composition: Chodl^em1Feyu/Chodl^em1Feyu; Pax7^{tm2.1(cre/ERT2)Fan}/Pax7⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

abnormal skeletal muscle satellite cell proliferation ( J:384967 )

• precocious activation with 5 fold higher percentage of EdU+ satellite cells

decreased skeletal muscle satellite cell proliferation ( J:384967 )

• in tibialis anterior (TA) muscles after induced Cre expression and CTX-induced muscle injury (7.1% vs 13.1% in controls)

abnormal skeletal muscle morphology ( J:384967 )

• following induced Cre-mediated recombination the percentage of satellite cells loosely associated with isolated single myofibers from EDL muscles is increased (11%) compared to controls where only 3% of satellite cells are not closely attached to myofibers

• following induced Cre-mediated recombination around 30% of satellite cells are found outside the basal lamina in the interstitial space in muscles compared to only around 5% in controls

decreased satellite cell number ( J:384967 )

• in tibialis anterior (TA) muscles after induced Cre expression and CTX-induced muscle injury

impaired skeletal muscle regeneration ( J:384967 )

• in tibialis anterior (TA) muscles after induced Cre expression and CTX-induced muscle injury

cellular

abnormal skeletal muscle satellite cell proliferation ( J:384967 )

• precocious activation with 5 fold higher percentage of EdU+ satellite cells

decreased skeletal muscle satellite cell proliferation ( J:384967 )

• in tibialis anterior (TA) muscles after induced Cre expression and CTX-induced muscle injury (7.1% vs 13.1% in controls)

Genotype
MGI:8402570

cn2

• Allelic Composition: Chodl^em1Feyu/Chodl^em1Feyu; Myod1^{tm2.1(icre)Glh}/Myod1⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

muscle

muscle phenotype ( J:384967 )

N • no abnormalities in muscle growth or muscle fiber composition

abnormal skeletal muscle morphology ( J:384967 )

• at P7 and P21, higher percentages of satellite cells are located outside the basal lamina compared to controls suggesting a defect in homing

decreased satellite cell number ( J:384967 )

• 52% reduction in the number of PAX7+ satellite cells per tibialis anterior area

• at 21 days post injury the number of satellite cells is reduced by 70%

• in aged mice there is a 37% reduction in the number of PAX7+ satellite cells compared to age matched controls

impaired skeletal muscle regeneration ( J:384967 )

• reduced muscle recovery rate following CTX-induced injury of tibialis anterior (TA) muscles at 21 days post injury

• however, at 7 days post injury ratio of injured to uninjured TA muscle mass is similar to controls

• at both 7 and 21 days post injury muscles contain smaller centrally nucleated myofibers and myofibers are smaller at 7 days post injury

• muscle recovery rate in aged mice is also reduced with fewer centrally nucleated myofibers in injured TA muscles at 5 days post infection