Phenotypes associated with this allele

• Allele Symbol: Ddx3x^tm1.1Dlsi
• Allele Name: targeted mutation 1.1, Debra L Silver
• Allele ID: MGI:8270660
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ddx3x^tm1.1Dlsi/Ddx3x^+ Emx1^tm1(cre)Krj/Emx1^+	involves: 129 * 129S2/SvPas * C57BL/6J * C57BL/6N	MGI:8272221
cn2	Ddx3x^tm1.1Dlsi/Y Emx1^tm1(cre)Krj/Emx1^+	involves: 129 * 129S2/SvPas * C57BL/6J * C57BL/6N	MGI:8351820
ot3	Ddx3x^tm1.1Dlsi/Y	involves: 129 * C57BL/6J * C57BL/6N	MGI:8351821

Genotype
MGI:8272221

cn1

• Allelic Composition: Ddx3x^tm1.1Dlsi/Ddx3x⁺; Emx1^tm1(cre)Krj/Emx1⁺
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6J * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

increased radial glial cell number ( J:376178 )

♀ • the number of SOX2+ radial glial cells is increased at E14.5, both in total numbers and as a percentage of all cells, indicating an increase in progenitor number in the developing cortex

♀ • however, the number of TBR2+ intermediate progenitors is not different and no significant differences in the percentage of deep layer neurons or in upper layer neurons in cortices are seen

decreased brain size ( J:376178 )

♀ • mild reduction in brain size of P0 females

abnormal cerebral cortex morphology ( J:376178 )

♀ • mice exhibit a mild 6% reduction in cortical area, indicating a mild microcephaly

♀ • however, cortical area is normal at E11.5, E14.5, and E16.5 and apoptosis is not apparent in cortices at any stage assessed

growth/size/body

microcephaly ( J:376178 )

♀ • mild

cellular

increased radial glial cell number ( J:376178 )

♀ • the number of SOX2+ radial glial cells is increased at E14.5, both in total numbers and as a percentage of all cells, indicating an increase in progenitor number in the developing cortex

♀ • however, the number of TBR2+ intermediate progenitors is not different and no significant differences in the percentage of deep layer neurons or in upper layer neurons in cortices are seen

Genotype
MGI:8351820

cn2

• Allelic Composition: Ddx3x^tm1.1Dlsi/Y; Emx1^tm1(cre)Krj/Emx1⁺
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6J * C57BL/6N

nervous system

increased forebrain apoptosis ( J:376178 )

♂ • apoptosis is not seen in cortices at E10.5, but is obvious at E11.5

abnormal cerebral cortex morphology ( J:376178 )

♂ • mice exhibit a 47% reduction in cortical area, indicating a profound microcephaly

♂ • a 37% reduction in cortical area is seen at E14.5, approximately 5 days after the -onset of onset of the cortex-specific cre expression

growth/size/body

microcephaly ( J:376178 )

♂

cellular

increased forebrain apoptosis ( J:376178 )

♂ • apoptosis is not seen in cortices at E10.5, but is obvious at E11.5

Genotype
MGI:8351821

ot3

• Allelic Composition: Ddx3x^tm1.1Dlsi/Y
• Genetic Background: involves: 129 * C57BL/6J * C57BL/6N

mortality/aging

lethality, incomplete penetrance ( J:376178 )

♂ • fewer than the expected numbers of males are recovered

growth/size/body

decreased body size ( J:376178 )

♂

nervous system

abnormal cerebral cortex morphology ( J:376178 )

♂ • mice exhibit a slight, but significant, 12% decrease in cortical area with apparent reduced body size

♂ • however, apoptosis is not present in cortices