Phenotypes associated with this allele

• Allele Symbol: Trap1^em1Iimcb
• Allele Name: endonuclease-mediated mutation 1, International Institute of Molecular and Cellular Biology in Warsaw
• Allele ID: MGI:8163545
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trap1^em1Iimcb/Trap1^em1Iimcb	B6.CBA-Trap1^em1Iimcb/Tar	MGI:8164244
ht2	Trap1^em1Iimcb/Trap1^+	B6.CBA-Trap1^em1Iimcb/Tar	MGI:8164245

Genotype
MGI:8164244

hm1

• Allelic Composition: Trap1^em1Iimcb/Trap1^em1Iimcb
• Genetic Background: B6.CBA-Trap1^em1Iimcb/Tar

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal social/conspecific interaction behavior ( J:361875 )

♂ • analysis of in-cohort sociability, which measures how much time each pair of animals living in the same cage spends together shows that males, but not females, have deficits in social interaction, spending less time with other familiar mice

abnormal social investigation ( J:361875 )

• in the three-chamber social approach test, both males and females show no preference for the unfamiliar mouse and explore the non-social object and the new mouse equally

cellular

abnormal neuron mitochondrial morphology ( J:361875 )

♂ • density of presynaptic mitochondria is lower in the CA1 hippocampus of males

♂ • however, the average size and volume of individual mitochondria is unchanged

nervous system

abnormal neuron mitochondrial morphology ( J:361875 )

♂ • density of presynaptic mitochondria is lower in the CA1 hippocampus of males

♂ • however, the average size and volume of individual mitochondria is unchanged

abnormal synaptic bouton morphology ( J:361875 )

♂ • density of presynaptic mitochondria is lower in the CA1 hippocampus of males

decreased dendritic spine density ( J:361875 )

♀ • females exhibit slightly reduced spine density in the CA1 region of the hippocampus and larger individual spines

increased dendritic spine density ( J:361875 )

♂ • males exhibit increased spine density in the CA1 region of the hippocampus, but spines are shorter and have decreased area

decreased dendritic spine length ( J:361875 )

♂ • dendritic spines in the CA1 region of the hippocampus are shorter in males

increased dendritic spine length ( J:361875 )

♀ • dendritic spines in the CA1 region of the hippocampus are larger in females

abnormal synaptic physiology ( J:361875 )

♂ • presynaptic mitochondria exhibit changes in metabolism, with males, but not females, showing decreased usage of succinate, the substrate for complex II and increased consumption of pyruvate + malate, substrates for complex I

♂ • mitochondria isolated from brain (cortex and hippocampus) exhibits a higher rate of respiration in the presence of pyruvate, glutamate, and malate

♂ • mitochondria isolated from cortex and hippocampus of males shows a trend towards decreased respiration in the presence of ascorbate and TMPD, the substrates for complex IV when complex I and III are inhibited

abnormal excitatory postsynaptic potential ( J:361875 )

• males exhibit significantly enhanced AMPAR-mediated synaptic responses recorded in response to monotonically increasing stimuli

• males exhibit significantly larger NMDARs-mediated synaptic responses

• females exhibit reduced NMDAR-mediated synaptic transmission

• however, no changes in the sensitivity of fEPSPs to AMPAR blocker DNQX are seen in either sex

decreased paired-pulse ratio ( J:361875 )

♂ • males, but not females, exhibit reduced average paired-pulse ratio for 25-ms and 50-ms interstimulus intervals indicating dysregulation of short-term synaptic plasticity

Genotype
MGI:8164245

ht2

• Allelic Composition: Trap1^em1Iimcb/Trap1⁺
• Genetic Background: B6.CBA-Trap1^em1Iimcb/Tar

behavior/neurological

abnormal social/conspecific interaction behavior ( J:361875 )

♂ • analysis of in-cohort sociability, which measures how much time each pair of animals living in the same cage spends together shows that males, but not females, have deficits in social interaction, spending less time with other familiar mice

cellular

abnormal neuron mitochondrial morphology ( J:361875 )

♂ • density of presynaptic mitochondria is lower in the CA1 hippocampus of males

nervous system

abnormal neuron mitochondrial morphology ( J:361875 )

♂ • density of presynaptic mitochondria is lower in the CA1 hippocampus of males

abnormal synaptic bouton morphology ( J:361875 )

♂ • density of presynaptic mitochondria is lower in the CA1 hippocampus of males

abnormal dendritic spine morphology ( J:361875 )

♀ • females exhibit increased spine density, length, head width, and area in the medial prefrontal cortex

increased dendritic spine density ( J:361875 )

• males and females exhibit increased spine density in the medial prefrontal cortex

increased dendritic spine length ( J:361875 )

♀ • females exhibit increased spine length

abnormal excitatory postsynaptic potential ( J:361875 )

♀ • females exhibit reduced NMDAR-mediated synaptic transmission