Phenotypes associated with this allele

• Allele Symbol: Sike1^em1Cya
• Allele Name: endonuclease-mediated mutation 1, Cyagen Biosciences
• Allele ID: MGI:7796137
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sike1^em1Cya/Sike1^em1Cya	C57BL/6N-Sike1^em1Cya/Cya	MGI:8217392

Genotype
MGI:8217392

hm1

• Allelic Composition: Sike1^em1Cya/Sike1^em1Cya
• Genetic Background: C57BL/6N-Sike1^em1Cya/Cya

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal enzyme/coenzyme level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 (particulate matter with a diameter of 2.5 micrometers or less) for 24 weeks, male mice show significantly higher hepatic aspartate transaminase (AST) and alanine transaminase (ALT) levels than PM2.5-challenged wild-type males

♂ • however, no significant differences in hepatic AST or ALT levels are noted after exposure to filtered air

increased interleukin level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show significantly higher Il1b and Il6 mRNA expression and IL1B and IL6 protein levels in liver samples than PM2.5-challenged wild-type males

abnormal tumor necrosis factor level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show significantly higher Tnf mRNA expression and TNF protein levels in liver samples than PM2.5-challenged wild-type males

increased cytokine level ( J:367804 )

♂ • after exposure to PM2.5 for 24 weeks, male mice show significantly higher mRNA expression of pro-inflammatory factors (Il1b, Il6, Tnf and Ifnb1) in hepatic tissues than PM2.5-challenged wild-type males

increased interferon level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show significantly higher Ifnb1 (IFN-beta) mRNA expression in liver samples than PM2.5-challenged wild-type males

increased susceptibility to injury ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show accelerated hepatic injury by H&E staining and significantly higher hepatic ALT and AST levels but no change in liver weight relative to PM2.5-challenged wild-type males

immune system

increased interleukin level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show significantly higher Il1b and Il6 mRNA expression and IL1B and IL6 protein levels in liver samples than PM2.5-challenged wild-type males

abnormal tumor necrosis factor level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show significantly higher Tnf mRNA expression and TNF protein levels in liver samples than PM2.5-challenged wild-type males

increased cytokine level ( J:367804 )

♂ • after exposure to PM2.5 for 24 weeks, male mice show significantly higher mRNA expression of pro-inflammatory factors (Il1b, Il6, Tnf and Ifnb1) in hepatic tissues than PM2.5-challenged wild-type males

increased interferon level ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show significantly higher Ifnb1 (IFN-beta) mRNA expression in liver samples than PM2.5-challenged wild-type males

liver/biliary system

abnormal liver physiology ( J:367804 )

♂ • after exposure to PM2.5 for 24 weeks, male mice show exacerbated liver injury and dysfunction with enhanced inflammation and oxidative stress and markedly upregulated activation of TBK1 and NF-kappaB relative to PM2.5-challenged wild-type males

cardiovascular system

increased mean systemic arterial blood pressure ( J:367804 )

♂ • after exposure to concentrated ambient PM2.5 for 24 weeks, male mice show a significantly higher mean blood pressure (MBP) than PM2.5-challenged wild-type males

♂ • however, no significant differences in MBP are noted after exposure to filtered air

cellular

oxidative stress ( J:367804 )

♂ • after exposure to PM2.5 for 24 weeks, male mice show significantly lower mRNA levels of genes encoding antioxidant enzymes (Hmox1, Gclc and Gclm), lower superoxide dismutase (SOD) activity, and higher malondialdehyde (MDA) levels in liver samples than PM2.5-challenged wild-type males

growth/size/body

growth/size/body region phenotype ( J:367804 )

♂N • after exposure to either filtered air or concentrated ambient PM2.5 for 24 weeks, male mice exhibit no significant body weight changes relative to similarly treated wild-type males