Phenotypes associated with this allele

• Allele Symbol: Clptm1^{tm1c(KOMP)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:7764843
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Clptm1^tm1c(KOMP)Wtsi/Clptm1^tm1c(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N * SJL/J	MGI:8259301

Genotype
MGI:8259301

hm1

• Allelic Composition: Clptm1^{tm1c(KOMP)Wtsi}/Clptm1^{tm1c(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6J * C57BL/6N * SJL/J
• Cell Lines: EPD0395_5_D10

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal inhibitory synapse morphology ( J:357551 )

• in culture, E18 hippocampal neurons transfected with Cre + DIO-GFP at 0 DIV using nucleofection and immunostained for surface GABA_AR gamma2 and for VGAT (an inhibitory presynaptic marker) at 14 DIV show significantly increased synaptic surface GABA_AR gamma2 intensity but no change in postsynaptic gamma2 puncta number

abnormal CNS synaptic transmission ( J:357551 )

• cultured hippocampal neurons and acute hippocampal slices transfected with Cre + DIO-GFP show increased inhibitory postsynaptic transmission

increased miniature inhibitory postsynaptic current amplitude ( J:357551 )

• in culture, E18 hippocampal neurons transfected with Cre + DIO-GFP at 0 DIV show a significant increase in mIPSC amplitude at 14 DIV, consistent with the observed increase in synaptic GABA_AR intensity

• in vivo, P0 newborns injected with AAV-Cre + AAV-DIO-GFP show a significantly increased mIPSC amplitude in hippocampal CA1 pyramidal neurons from acute brain slices at P15-19

• however, cultured hippocampal neurons and acute hippocampal slices transfected with Cre + DIO-GFP show no significant changes in mIPSC decay time or mIPSC frequency, consistent with normal postsynaptic GABA_AR cluster density