Phenotypes associated with this allele

• Allele Symbol: Supt16^em1Hguo
• Allele Name: endonuclease-mediated mutation 1, Hong Guo
• Allele ID: MGI:7620382
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Supt16^em1Hguo/Supt16^em1Hguo	Not Specified	MGI:7620387
ht2	Supt16^em1Hguo/Supt16^+	Not Specified	MGI:7620390

Genotype
MGI:7620387

hm1

• Allelic Composition: Supt16^em1Hguo/Supt16^em1Hguo
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:344671 )

• early embryonic lethality

Genotype
MGI:7620390

ht2

• Allelic Composition: Supt16^em1Hguo/Supt16⁺
• Genetic Background: Not Specified

behavior/neurological

abnormal object recognition memory ( J:344671 )

• recognition memory using a novel object recognition test; mice do not show any preference for exploring either familiar or novel objects in the novel object recognition test

impaired spatial learning ( J:344671 )

• in the Morris water maze, mice show a difference in the escape latency after 1 day of training, indicating impaired spatial learning

abnormal spatial reference memory ( J:344671 )

• in the probe trial of the Morris water maze, mice show a decrease in platform crossing and quadrant crossing

• however, no differences in the Y-maze are seen

increased thigmotaxis ( J:344671 )

• mice show increased anxiety, spending less time in the open field center

decreased social novelty preference ( J:344671 )

• in the social novelty test, mice show no difference in chambers containing an unfamiliar or a familiar mouse

decreased social investigation ( J:344671 )

• in the three-chamber social test, mice show no preference for either the chamber containing an unfamiliar mouse or an empty chamber

nervous system

abnormal dendrite morphology ( J:344671 )

• the maximum dendritic span is smaller

decreased dendritic spine density ( J:344671 )

• total spine density is decreased

decreased dendritic spine length ( J:344671 )

• neuronal dendrite length is decreased in the cortex

decreased dendritic spine number ( J:344671 )

• basal dendrites and apical dendrites of neurons in CA1 zone are reduced

• the number of dendrites is decreased

decreased neuron number ( J:344671 )

• mice show reduced number of neurons and dendritic spines in the brain

• expression of NeuN is decreased in the cerebral cortex and the number of neurons is decreased in the hippocampal CA1 and DG zones

• however, no difference is seen in NeuN+ neurons at the CA3 zone

abnormal nervous system electrophysiology ( J:344671 )

• analysis of local field potentials, mice show decreased power of theta and high-gamma oscillations in CA1 area of hippocampus when freely moving in the open field

abnormal neuronal stem cell physiology ( J:344671 )

• mouse neural stem cells show an increase in the DNA damage marker gammaH2A.X, indicating increased apoptosis

decreased neuronal stem cell self-renewal ( J:344671 )

• mouse neural stem cells in culture form smaller neurosphere structures, show decreased proliferation