Phenotypes associated with this allele

• Allele Symbol: Actrt3^em2Cya
• Allele Name: endonuclease-mediated mutation 2, Cyagen Biosciences
• Allele ID: MGI:7582154
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Actrt3^em2Cya/Actrt3^em2Cya Tg(Stra8-icre)1Reb/0	involves: C57BL/6J * FVB/NJ	MGI:8398193

Genotype
MGI:8398193

cn1

• Allelic Composition: Actrt3^em2Cya/Actrt3^em2Cya; Tg(Stra8-icre)1Reb/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

teratozoospermia ( J:390202 )

♂ • elevated rate of sperm teratospermia

abnormal sperm head morphology ( J:390202 )

♂ • sperm exhibit a loss of the characteristic hook-shaped curvature of the sperm head, showing a blunt, rounded morphology

abnormal acrosome morphology ( J:390202 )

♂ • acrosomal structure is aberrant

detached acrosome ( J:390202 )

♂ • acrosome is detached from the nuclear membrane

reduced male fertility ( J:390202 )

♂ • males exhibit reduced fertility, producing a decreased number of offspring

impaired fertilization ( J:390202 )

♂ • intracytoplasmic sperm injection with mutant sperm leads to only sporadic cleavage of oocytes by day 2, and these cleaved embryos arrest at the 2-cell or 4-cell stage indicating fertilization and embryonic arrest

♂ • sperm exhibit impaired ability to fertilize artificial oocyte activated oocytes

♂ • sperm treated with SrCl2 combined with ionomycin significantly rescue the fertilization capability of sperm

cellular

teratozoospermia ( J:390202 )

♂ • elevated rate of sperm teratospermia

abnormal sperm head morphology ( J:390202 )

♂ • sperm exhibit a loss of the characteristic hook-shaped curvature of the sperm head, showing a blunt, rounded morphology

abnormal acrosome morphology ( J:390202 )

♂ • acrosomal structure is aberrant

detached acrosome ( J:390202 )

♂ • acrosome is detached from the nuclear membrane