Phenotypes associated with this allele

• Allele Symbol: Polr1a^{tm1c(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:7432631
• Summary: 17 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Foxg1^tm1.1(cre)Ddmo/0 Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^em1Knwea/Polr1a^tm1c(EUCOMM)Hmgu	involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N	MGI:7526484
cn2	Foxg1^tm1.1(cre)Ddmo/Foxg1^+ Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1d(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu	involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N	MGI:7526488
cn3	Foxg1^tm1.1(cre)Ddmo/Foxg1^+ Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1a(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu	involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N	MGI:7526487
cn4	Foxg1^tm1.1(cre)Ddmo/Foxg1^+ Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1c(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu	involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N	MGI:7526489
cn5	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^em1Knwea/Polr1a^tm1c(EUCOMM)Hmgu Tg(Wnt1-cre/Esr1*)10Rth/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6N	MGI:7526471
cn6	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^em1Knwea/Polr1a^tm1c(EUCOMM)Hmgu Tg(Sox10-cre)1Wdr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6N * CBA	MGI:7526470
cn7	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1a(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Mef2c-cre)2Blk/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7526459
cn8	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1d(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Mef2c-cre)2Blk/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7526462
cn9	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1c(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Mef2c-cre)2Blk/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7526463
cn10	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1a(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Wnt1-cre/Esr1*)10Rth/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7526475
cn11	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1d(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Wnt1-cre/Esr1*)10Rth/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7526477
cn12	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1c(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Wnt1-cre/Esr1*)10Rth/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7526481
cn13	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1d(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Sox10-cre)1Wdr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N * CBA	MGI:7526466
cn14	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1a(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Sox10-cre)1Wdr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N * CBA	MGI:7526465
cn15	Polr1a^tm1c(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/Gt(ROSA)26Sor^+ Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * 129X1/SvJ * C57BL/6J * C57BL/6N	MGI:8408504
cn16	Polr1a^tm1c(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * C57BL/6J * C57BL/6N	MGI:8408503
cn17	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Polr1a^tm1c(EUCOMM)Hmgu/Polr1a^tm1c(EUCOMM)Hmgu Tg(Sox10-cre)1Wdr/0	Not Specified	MGI:7526467

Genotype
MGI:7526484

cn1

• Allelic Composition: Foxg1^{tm1.1(cre)Ddmo}/0; Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^em1Knwea/Polr1a^{tm1c(EUCOMM)Hmgu}
• Genetic Background: involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

telencephalon hypoplasia ( J:335489 )

• in E14 and E17 embryos

• normal in E12 embryos

Genotype
MGI:7526488

cn2

• Allelic Composition: Foxg1^{tm1.1(cre)Ddmo}/Foxg1⁺; Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1d(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}
• Genetic Background: involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N

nervous system

telencephalon hypoplasia ( J:335489 )

• in E12, E14 and E17 embryos

Genotype
MGI:7526487

cn3

• Allelic Composition: Foxg1^{tm1.1(cre)Ddmo}/Foxg1⁺; Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1a(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}
• Genetic Background: involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N

nervous system

telencephalon hypoplasia ( J:335489 )

• in E12, E14 and E17 embryos

Genotype
MGI:7526489

cn4

• Allelic Composition: Foxg1^{tm1.1(cre)Ddmo}/Foxg1⁺; Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1c(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}
• Genetic Background: involves: 129S1/Sv * 129T/Sv * 129X1/SvJ * C57BL/6N

nervous system

telencephalon hypoplasia ( J:335489 )

• in E12, E14 and E17 embryos

Genotype
MGI:7526471

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^em1Knwea/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Wnt1-cre/Esr1*)10Rth/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6N

mortality/aging

lethality throughout fetal growth and development ( J:335489 )

craniofacial

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

facial cleft ( J:335489 )

growth/size/body

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

facial cleft ( J:335489 )

cardiovascular system

cardiovascular system phenotype ( J:335489 )

N • normal outflow tract septation in E12 embryos

Genotype
MGI:7526470

cn6

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^em1Knwea/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Sox10-cre)1Wdr/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6N * CBA

craniofacial

midline cleft upper lip ( J:335489 )

• median lip notches in some E14-E15 embryos

cleft palate ( J:335489 )

• in some E14-E15 embryos

facial cleft ( J:335489 )

• in some E14-E15 embryos

digestive/alimentary system

cleft palate ( J:335489 )

• in some E14-E15 embryos

growth/size/body

midline cleft upper lip ( J:335489 )

• median lip notches in some E14-E15 embryos

cleft palate ( J:335489 )

• in some E14-E15 embryos

facial cleft ( J:335489 )

• in some E14-E15 embryos

Genotype
MGI:7526459

cn7

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1a(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Mef2c-cre)2Blk/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

cardiovascular system

ventricular septal defect ( J:335489 )

• in E12 embryos

enlarged heart ( J:335489 )

• in newborns, owing to blood pooling

heart right ventricle hypoplasia ( J:335489 )

• in E12 embryos

abnormal heart right ventricle outflow tract morphology ( J:335489 )

• shorter in E10 embryos

absent heart right ventricle ( J:335489 )

• newborn hearts have only single ventricle

growth/size/body

enlarged heart ( J:335489 )

• in newborns, owing to blood pooling

Genotype
MGI:7526462

cn8

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1d(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Mef2c-cre)2Blk/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

cardiovascular system

ventricular septal defect ( J:335489 )

• in E12 embryos

enlarged heart ( J:335489 )

• in newborns, owing to blood pooling

heart right ventricle hypoplasia ( J:335489 )

• in E12 embryos

abnormal heart right ventricle outflow tract morphology ( J:335489 )

• shorter in E10 embryos

absent heart right ventricle ( J:335489 )

• newborn hearts have only single ventricle

growth/size/body

enlarged heart ( J:335489 )

• in newborns, owing to blood pooling

Genotype
MGI:7526463

cn9

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1c(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Mef2c-cre)2Blk/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

cardiovascular system

ventricular septal defect ( J:335489 )

• in E12 embryos

enlarged heart ( J:335489 )

• in newborns, owing to blood pooling

heart right ventricle hypoplasia ( J:335489 )

• in E12 embryos

abnormal heart right ventricle outflow tract morphology ( J:335489 )

• shorter in E10 embryos

absent heart right ventricle ( J:335489 )

• newborn hearts have only single ventricle

growth/size/body

enlarged heart ( J:335489 )

• in newborns, owing to blood pooling

Genotype
MGI:7526475

cn10

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1a(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Wnt1-cre/Esr1*)10Rth/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

cardiovascular system

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

persistent truncus arteriosus ( J:335489 )

• in E12 embryos

decreased heart left ventricle wall thickness ( J:335489 )

• in E12.5 embryos

increased vascular permeability ( J:335489 )

• diffuse vascular leakage in E11.5 embryos

craniofacial

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

embryo

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

growth/size/body

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

mortality/aging

lethality throughout fetal growth and development ( J:335489 )

embryonic lethality during organogenesis, complete penetrance ( J:335489 )

Genotype
MGI:7526477

cn11

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1d(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Wnt1-cre/Esr1*)10Rth/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

cardiovascular system

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

persistent truncus arteriosus ( J:335489 )

• in E12 embryos

decreased heart left ventricle wall thickness ( J:335489 )

• in E12.5 embryos

increased vascular permeability ( J:335489 )

• diffuse vascular leakage in E11.5 embryos

craniofacial

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

embryo

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

growth/size/body

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

mortality/aging

lethality throughout fetal growth and development ( J:335489 )

embryonic lethality during organogenesis, complete penetrance ( J:335489 )

Genotype
MGI:7526481

cn12

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1c(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Wnt1-cre/Esr1*)10Rth/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

cardiovascular system

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

persistent truncus arteriosus ( J:335489 )

• in E12 embryos

decreased heart left ventricle wall thickness ( J:335489 )

• in E12.5 embryos

increased vascular permeability ( J:335489 )

• diffuse vascular leakage in E11.5 embryos

craniofacial

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

embryo

pharyngeal arch artery hypoplasia ( J:335489 )

• in E11.5 embryos

growth/size/body

flat forehead ( J:335489 )

• starting in E9.5 embryos, worsening with age

mortality/aging

lethality throughout fetal growth and development ( J:335489 )

embryonic lethality during organogenesis, complete penetrance ( J:335489 )

Genotype
MGI:7526466

cn13

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1d(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Sox10-cre)1Wdr/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N * CBA

cardiovascular system

cardiovascular system phenotype ( J:335489 )

• normal outflow tract septation in E9.5 embryos

craniofacial

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

temporal bone squamous part hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft palate ( J:335489 )

• in E16 and E17 embryos

facial cleft ( J:335489 )

• in E16 and E17 embryos

digestive/alimentary system

cleft palate ( J:335489 )

• in E16 and E17 embryos

growth/size/body

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft palate ( J:335489 )

• in E16 and E17 embryos

facial cleft ( J:335489 )

• in E16 and E17 embryos

respiratory system

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

skeleton

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

temporal bone squamous part hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

Genotype
MGI:7526465

cn14

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1a(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Sox10-cre)1Wdr/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N * CBA

cardiovascular system

cardiovascular system phenotype ( J:335489 )

N • normal outflow tract septation in E9.5 embryos

craniofacial

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

temporal bone squamous part hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft palate ( J:335489 )

• in E16 and E17 embryos

facial cleft ( J:335489 )

• in E16 and E17 embryos

digestive/alimentary system

cleft palate ( J:335489 )

• in E16 and E17 embryos

growth/size/body

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft palate ( J:335489 )

• in E16 and E17 embryos

facial cleft ( J:335489 )

• in E16 and E17 embryos

respiratory system

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

skeleton

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

temporal bone squamous part hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

Genotype
MGI:8408504

cn15

• Allelic Composition: Polr1a^{tm1c(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor⁺; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S4/SvJae * 129X1/SvJ * C57BL/6J * C57BL/6N
• Cell Lines: HEPD0779_7_B03

craniofacial

abnormal craniofacial morphology ( J:381064 )

• at E12, brightfield images of embryos (tamoxifen-induced at E9.5) show craniofacial malformations

Genotype
MGI:8408503

cn16

• Allelic Composition: Polr1a^{tm1c(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6J * C57BL/6N
• Cell Lines: HEPD0779_7_B03

craniofacial

abnormal craniofacial morphology ( J:381064 )

• at E12.5, embryos (tamoxifen-injected at E9.5) exhibit craniofacial abnormalities

abnormal cranial cartilage development ( J:381064 )

• at E12.5, in situ hybridization for Sox9 (a master regulator of chondrogenesis) and Sox10 (a marker of developing peripheral nervous system) and immunostaining for SOX9 and TUJ1 (TUBB3A; neuron-specific class III beta-tubulin) indicate a decreased SOX9 signal in the mandibular region, consistent with reduced cartilage development

abnormal craniofacial development ( J:381064 )

• compositional analysis of single cell transcriptomics data from E12.5 embryos (tamoxifen-injected at E9.5) shows a significant reduction of several ectomesenchymal clusters, without affecting neuroglial progeny

• at E12.5, embryos (tamoxifen-injected at E9.5) exhibit reduced facial mesenchymal condensations and impaired cartilage development

• 3D visualizations based on microCT data indicate that while ectomesenchymal chondrogenic condensations are severely reduced at E12.5, trigeminal ganglia are not significantly affected, indicating that facial skeletogenesis is specifically disrupted due to reduced rRNA transcription and ribosome biogenesis

fragmented Meckel's cartilage ( J:381064 )

• at E12.5, embryos (tamoxifen-injected at E9.5) exhibit a disrupted Meckels cartilage, presenting as two discrete non-contiguous proximal and distal elements rather than a continuous structure

• however, the branches of the neural crest-derived trigeminal ganglion are relatively less affected, and the mandibular nerve now traverses the gap between the non-contiguous Meckels cartilage elements

abnormal palatal shelf morphology ( J:381064 )

abnormal face development ( J:381064 )

• at E12.5, embryos (tamoxifen-injected at E9.5) exhibit reduced facial mesenchymal condensations

growth/size/body

abnormal palatal shelf morphology ( J:381064 )

abnormal face development ( J:381064 )

• at E12.5, embryos (tamoxifen-injected at E9.5) exhibit reduced facial mesenchymal condensations

skeleton

fragmented Meckel's cartilage ( J:381064 )

• at E12.5, embryos (tamoxifen-injected at E9.5) exhibit a disrupted Meckels cartilage, presenting as two discrete non-contiguous proximal and distal elements rather than a continuous structure

• however, the branches of the neural crest-derived trigeminal ganglion are relatively less affected, and the mandibular nerve now traverses the gap between the non-contiguous Meckels cartilage elements

abnormal cranial cartilage development ( J:381064 )

• at E12.5, in situ hybridization for Sox9 (a master regulator of chondrogenesis) and Sox10 (a marker of developing peripheral nervous system) and immunostaining for SOX9 and TUJ1 (TUBB3A; neuron-specific class III beta-tubulin) indicate a decreased SOX9 signal in the mandibular region, consistent with reduced cartilage development

digestive/alimentary system

abnormal palatal shelf morphology ( J:381064 )

Genotype
MGI:7526467

cn17

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Polr1a^{tm1c(EUCOMM)Hmgu}/Polr1a^{tm1c(EUCOMM)Hmgu}; Tg(Sox10-cre)1Wdr/0
• Genetic Background: Not Specified

cardiovascular system

cardiovascular system phenotype ( J:335489 )

• normal outflow tract septation in E9.5 embryos

craniofacial

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

temporal bone squamous part hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft palate ( J:335489 )

• in E16 and E17 embryos

facial cleft ( J:335489 )

• in E16 and E17 embryos

digestive/alimentary system

cleft palate ( J:335489 )

• in E16 and E17 embryos

growth/size/body

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft palate ( J:335489 )

• in E16 and E17 embryos

facial cleft ( J:335489 )

• in E16 and E17 embryos

respiratory system

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

skeleton

frontal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos

temporal bone squamous part hypoplasia ( J:335489 )

• in E16 and E17 embryos

cleft chin ( J:335489 )

• mandibular cleft in E14-E17 embryos

short mandible ( J:335489 )

• in E17 embryos

nasal bone hypoplasia ( J:335489 )

• in E16 and E17 embryos